Decorin Protein (DCN) (AA 1-359) (GST tag)
(4 references)-
- Target See all Decorin (DCN) Proteins
- Decorin (DCN)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-359
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This Decorin protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- DCN (Human) Recombinant Protein (P01)
- Sequence
- MKATIILLLL AQVSWAGPFQ QRGLFDFMLE DEASGIGPEV PDDRDFEPSL GPVCPFRCQC HLRVVQCSDL GLDKVPKDLP PDTTLLDLQN NKITEIKDGD FKNLKNLHAL ILVNNKISKV SPGAFTPLVK LERLYLSKNQ LKELPEKMPK TLQELRAHEN EITKVRKVTF NGLNQMIVIE LGTNPLKSSG IENGAFQGMK KLSYIRIADT NITSIPQGLP PSLTELHLDG NKISRVDAAS LKGLNNLAKL GLSFNSISAV DNGSLANTPH LRELHLDNNK LTRVPGGLAE HKYIQVVYLH NNNISVVGSS DFCPPGHNTK KASYSGVSLF SNPVQYWEIQ PSTFRCVYVR SAIQLGNYK
- Characteristics
- Human DCN full-length ORF ( AAH05322, 1 a.a. - 359 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Decorin (DCN) protein (His tag)
DCN Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Decorin (DCN) (AA 36-195) protein (His-IF2DI Tag)DCN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Decorin (DCN) (AA 35-354) protein (His tag)DCN Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Decorin (DCN) (AA 31-354) protein (His tag)Crystallography grade DCN Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "The anti-metastatic activity of collagenase-2 in breast cancer cells is mediated by a signaling pathway involving decorin and miR-21." in: Oncogene, Vol. 33, Issue 23, pp. 3054-63, (2014) (PubMed).
: "Decorin is a novel VEGFR-2-binding antagonist for the human extravillous trophoblast." in: Molecular endocrinology (Baltimore, Md.), Vol. 25, Issue 8, pp. 1431-43, (2011) (PubMed).
: "Serpina3n attenuates granzyme B-mediated decorin cleavage and rupture in a murine model of aortic aneurysm." in: Cell death & disease, Vol. 2, pp. e209, (2011) (PubMed).
: "Granzyme B contributes to extracellular matrix remodeling and skin aging in apolipoprotein E knockout mice." in: Experimental gerontology, Vol. 46, Issue 6, pp. 489-99, (2011) (PubMed).
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: "The anti-metastatic activity of collagenase-2 in breast cancer cells is mediated by a signaling pathway involving decorin and miR-21." in: Oncogene, Vol. 33, Issue 23, pp. 3054-63, (2014) (PubMed).
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- Target
- Decorin (DCN)
- Alternative Name
- DCN (DCN Products)
- Synonyms
- CSCD Protein, DSPG2 Protein, PG40 Protein, PGII Protein, PGS2 Protein, SLRR1B Protein, DC Protein, mDcn Protein, cscd Protein, dspg2 Protein, pg40 Protein, pgii Protein, pgs2 Protein, slrr1b Protein, decorin Protein, decorin L homeolog Protein, DCN Protein, Dcn Protein, dcn.L Protein, dcn Protein
- Background
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Full Gene Name: decorin
Synonyms: CSCD,DSPG2,PG40,PGII,PGS2,SLRR1B - Gene ID
- 1634
- Pathways
- Glycosaminoglycan Metabolic Process
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