Proton ATPase (AA 826-838) antibody

Catalog No. ABIN1742216

Product Details

Target: Proton ATPase

Binding Specificity: AA 826-838

Reactivity: Hamster, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Specificity: Specific for the alpha1 116kDa subunit.

Purification: antiserum

Immunogen: Synthetic peptide FSFEHIREGKFDE (826-838 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100
IHC-P: 1 : 100

Comment:

WB: The proton pump aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples. ICC: Methanol fixation is recommended.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References

Gouzy, Larrouy-Maumus, Bottai, Levillain, Dumas, Wallach, Caire-Brandli, de Chastellier, Wu, Poincloux, Brosch, Guerquin-Kern, Schnappinger, Sório de Carvalho, Poquet, Neyrolles: "Mycobacterium tuberculosis exploits asparagine to assimilate nitrogen and resist acid stress during infection." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003928, (2014) (PubMed).

Yao, de la Iglesia, Bajjalieh: "Loss of the SV2-like protein SVOP produces no apparent deficits in laboratory mice." in: PLoS ONE, Vol. 8, Issue 7, pp. e68215, (2013) (PubMed).

Target Details

Target: Proton ATPase