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psaB antibody

psaB Reactivity: Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Nicotiana tabacum WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN488670
  • Target
    psaB (psi-B Core Subunit of Photosystem I (psaB))
    Reactivity
    Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Nicotiana tabacum
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Western Blotting (WB)
    Cross-Reactivity (Details)
    Peptide target used to elicit this antibody is well conserved in all photoautotrophs except some cyanobacteria, some red algae and Cyanophora paradoxa, which contain a conserved substitution of a valine to an isoleucine. The performance of the antibodies has been confirmed against taxa containing both the valine andisoleucine variants.
    Characteristics
    Expected / apparent Molecular Weight of the Antigene: 82.7 / 55-60 kDa
    Purification
    serum
    Immunogen
    KLH-conjugated synthetic peptide derived from known PsaB sequences including Arabidopsis thaliana P56767, AtCg00340
  • Application Notes
    Recommended Dilution: 1 : 1000 with standard ECL (WB).
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 200 µL, of sterile water.
    Handling Advice
    Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Storage
    -20 °C
  • Lintala, Schuck, Thormählen, Jungfer, Weber, Weber, Geigenberger, Soll, Bölter, Mulo: "Arabidopsis tic62 trol mutant lacking thylakoid-bound ferredoxin-NADP+ oxidoreductase shows distinct metabolic phenotype." in: Molecular plant, Vol. 7, Issue 1, pp. 45-57, (2014) (PubMed).

    Mustila, Allahverdiyeva, Isojärvi, Aro, Eisenhut: "The bacterial-type [4Fe-4S] ferredoxin 7 has a regulatory function under photooxidative stress conditions in the cyanobacterium Synechocystis sp. PCC 6803." in: Biochimica et biophysica acta, Vol. 1837, Issue 8, pp. 1293-304, (2014) (PubMed).

    Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).

    Krech, Ruf, Masduki, Thiele, Bednarczyk, Albus, Tiller, Hasse, Schöttler, Bock: "The plastid genome-encoded Ycf4 protein functions as a nonessential assembly factor for photosystem I in higher plants." in: Plant physiology, Vol. 159, Issue 2, pp. 579-91, (2012) (PubMed).

  • Target
    psaB (psi-B Core Subunit of Photosystem I (psaB))
    Synonyms
    PSI P700 apoprotein A2 antibody, psaB antibody
    Background
    AGI Code: ATCG00340
    Photosystem I (PSI) of chloroplasts is a multisubunit membrane-protein complex that catalyzes the electron transfer from the reduced plastocyanin (or cytochrome c6) in the thylakoid lumen to the oxidized ferredoxin (or flavodoxin) in the chloroplast stroma. PsaB is a core protein of PSI complex. Synonymes: Photosystem I P700 chlorophyll a apoprotein A2, PSI-B
    Molecular Weight
    expected: 82.7 kDa, apparent: 55-60 kDa
    UniProt
    P56767
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