FNDC5 Protein (AA 1-137) (GST tag)
(3 references)-
- Target See all FNDC5 Proteins
- FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-137
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This FNDC5 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- FNDC5 (Human) Recombinant Protein (P01)
- Sequence
- MLRFIQEVNT TTRSCALWDL EEDTEYIVHV QAISIQGQSP ASEPVLFKTP REAEKMASKN KDEVTMKEMG RNQQLRTGEV LIIVVVLFMW AGVIALFCRQ YDIIKDNEPN NNKEKTKSAS ETSTPEHQGG GLLRSKI
- Characteristics
- Human FNDC5 full-length ORF ( NP_715637.1, 1 a.a. - 137 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Fibronectin Type III Domain Containing 5 (FNDC5) (AA 32-143) protein (His-IF2DI Tag)
FNDC5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 15-127) protein (His tag)FNDC5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 14-78) protein (GST tag)FNDC5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Fibronectin Type III Domain Containing 5 (FNDC5) protein (His tag)FNDC5 Origin: Human, Mouse, Rat Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 32-152) protein (His tag)Crystallography grade FNDC5 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 29-149) protein (His tag)Crystallography grade FNDC5 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 32-143) protein (rho-1D4 tag)Crystallography grade FNDC5 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 32-212) protein (rho-1D4 tag)Crystallography grade FNDC5 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 29-140) protein (rho-1D4 tag)Crystallography grade FNDC5 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 29-209) protein (rho-1D4 tag)Crystallography grade FNDC5 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Browning of white fat: does irisin play a role in humans?" in: The Journal of endocrinology, Vol. 222, Issue 1, pp. R25-38, (2014) (PubMed).
: "Irisin and FNDC5 in retrospect: An exercise hormone or a transmembrane receptor?" in: Adipocyte, Vol. 2, Issue 4, pp. 289-93, (2013) (PubMed).
: "A PGC1-?-dependent myokine that drives brown-fat-like development of white fat and thermogenesis." in: Nature, Vol. 481, Issue 7382, pp. 463-8, (2012) (PubMed).
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: "Browning of white fat: does irisin play a role in humans?" in: The Journal of endocrinology, Vol. 222, Issue 1, pp. R25-38, (2014) (PubMed).
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- Target
- FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))
- Alternative Name
- FNDC5 (FNDC5 Products)
- Synonyms
- FRCP2 Protein, irisin Protein, 1500001L03Rik Protein, AI836596 Protein, C87088 Protein, PeP Protein, Pxp Protein, fndc5 Protein, si:dkey-264p5.1 Protein, zgc:158684 Protein, fibronectin type III domain containing 5 Protein, fibronectin type III domain containing 5b Protein, FNDC5 Protein, Fndc5 Protein, fndc5b Protein
- Background
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Full Gene Name: fibronectin type III domain containing 5
Synonyms: FRCP2 - Gene ID
- 252995
- NCBI Accession
- NM_153756
- Pathways
- Hormone Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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