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PRNP antibody

PRNP Reactivity: Human WB Host: Mouse Monoclonal EM-20 unconjugated
Catalog No. ABIN349704
  • Target See all PRNP Antibodies
    PRNP (Prion Protein (PRNP))
    Reactivity
    • 82
    • 33
    • 25
    • 11
    • 9
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 83
    • 12
    • 2
    • 2
    Mouse
    Clonality
    • 74
    • 25
    Monoclonal
    Conjugate
    • 48
    • 7
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    This PRNP antibody is un-conjugated
    Application
    • 56
    • 32
    • 27
    • 26
    • 16
    • 16
    • 8
    • 7
    • 7
    • 6
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB)
    Specificity
    The mouse monoclonal antibody EM-20 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein.
    Cross-Reactivity (Details)
    Human
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Recombinant human prion protein
    Clone
    EM-20
    Isotype
    IgG2a
    Top Product
    Discover our top product PRNP Primary Antibody
  • Application Notes
    Western blotting: Recommended dilution: 0.5 μg/mL, non-reducing conditions are essential.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Dvorakova, Vranac, Janouskova, ?ernilec, Koren, Lukan, Nováková, Matej, Holada, ?urin Šerbec: "Detection of the GPI-anchorless prion protein fragment PrP226* in human brain." in: BMC neurology, Vol. 13, pp. 126, (2013) (PubMed).

  • Target
    PRNP (Prion Protein (PRNP))
    Abstract
    PRNP Products
    Synonyms
    ASCR antibody, AltPrP antibody, CD230 antibody, CJD antibody, GSS antibody, KURU antibody, PRIP antibody, PrP antibody, PrP27-30 antibody, PrP33-35C antibody, PrPc antibody, p27-30 antibody, Prn antibody, Prn-p antibody, prp antibody, cjd antibody, prion antibody, prp(c) antibody, MGC84114 antibody, PRP antibody, PRPC antibody, Prp antibody, SIP antibody, PRNP antibody, AA960666 antibody, AI325101 antibody, PrP antibody, PrPC antibody, PrPSc antibody, Prn-i antibody, Sinc antibody, prP27-30 antibody, prP33-35C antibody, PrP-1 antibody, PrP1 antibody, prnprs1 antibody, stPrP-1 antibody, zgc:123004 antibody, prion protein antibody, prion protein S homeolog antibody, prion protein b antibody, PRNP antibody, Prnp antibody, prnp.S antibody, prnpb antibody
    Background
    Prion protein,CD230 / human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. CD230 / PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.,Major prion protein, PrP, PrP27-30, PrP33-35C, ASCR, CD230, PRNP, CJD, KURU, PRIP, PRPC
    Gene ID
    5621
    UniProt
    P04156
    Pathways
    Transition Metal Ion Homeostasis, Activated T Cell Proliferation
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