IL-6 ELISA Kit

Catalog No. ABIN411303

Product Details IL-6 ELISA Kit

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Binding Specificity: AA 29-212

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 4.69-300 pg/mL

Minimum Detection Limit: 4.69 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-6

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: P29-M212

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <0.3pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: P29-M212

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: human IL-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-6 has been precoated onto 96-well plates. Standards(E.coli, P29-M212) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-6 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.75pg/mL, 9.38pg/mL, 4.69pg/mL human IL-6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-6 standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 16.3, Standard deviation: 0.8, CV(%): 4.9
• Sample 2: n=16, Mean(pg/ml): 98, Standard deviation: 2.3, CV(%): 2.3
• Sample 3: n=16, Mean(pg/ml): 179, Standard deviation: 2.3, CV(%): 2.3,
• Sample 1: n=24, Mean(pg/ml): 18.2, Standard deviation: 1.0, CV(%): 5.5
• Sample 2: n=24, Mean(pg/ml): 99, Standard deviation: 3.6, CV(%): 3.6
• Sample 3: n=24, Mean(pg/ml): 185, Standard deviation: 5.7, CV(%): 3.1

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for IL-6 ELISA Kit (ABIN411303)

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Khan, Haseeb, Ansari, Haqqi: "A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1β-induced activation of AP-1 in human OA chondrocytes." in: Scientific reports, Vol. 7, pp. 43789, (2018) (PubMed).

Ma, Sun, Zhang, Zhang, Yao: "Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells." in: International journal of molecular medicine, Vol. 40, Issue 1, pp. 31-38, (2018) (PubMed).

He, Le, Frediani, Winterberg, Jin, Liverman, Hernandez, Cleeton, Vos: "Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA." in: Pediatric transplantation, Vol. 21, Issue 6, (2018) (PubMed).

Ünver, Deveci, Kırkıl, Telo, Kaman, Kuluöztürk: "Serum Heat Shock Protein Levels and the Relationship of Heat Shock Proteins with Various Parameters in Chronic Obstructive Pulmonary Disease Patients." in: Turkish thoracic journal, Vol. 17, Issue 4, pp. 153-159, (2018) (PubMed).

Qin, Yan, Wang, Xu, Wang, Zhu, Shi, Li, Zhang, Chen: "Cancer-associated Fibroblast-derived IL-6 Promotes Head and Neck Cancer Progression via the Osteopontin-NF-kappa B Signaling Pathway." in: Theranostics, Vol. 8, Issue 4, pp. 921-940, (2018) (PubMed).

Wen, Gan, Li, Sun, Xiong, Xia: "Safety of Low-calcium Dialysate and its Effects on Coronary Artery Calcification in Patients Undergoing Maintenance Hemodialysis." in: Scientific reports, Vol. 8, Issue 1, pp. 5941, (2018) (PubMed).

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Fallah, Feizi, Hashemipour, Kelishadi: "Effect of fermented camel milk on glucose metabolism, insulin resistance, and inflammatory biomarkers of adolescents with metabolic syndrome: A double-blind, randomized, crossover trial." in: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 23, pp. 32, (2018) (PubMed).

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Csősz, Lábiscsák, Kalló, Márkus, Emri, Szabó, Tar, Tőzsér, Kiss, Márton: "Proteomics investigation of OSCC-specific salivary biomarkers in a Hungarian population highlights the importance of identification of population-tailored biomarkers." in: PLoS ONE, Vol. 12, Issue 5, pp. e0177282, (2017) (PubMed).

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Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL6 (IL6 Products)

Synonyms: BSF2 ELISA Kit, HGF ELISA Kit, HSF ELISA Kit, IFNB2 ELISA Kit, IL-6 ELISA Kit, Il-6 ELISA Kit, ILg6 ELISA Kit, Ifnb2 ELISA Kit, il6 ELISA Kit, CHIL-6 ELISA Kit, interleukin 6 ELISA Kit, interleukin-6 ELISA Kit, IL6 ELISA Kit, Il6 ELISA Kit, il-6 ELISA Kit, IL-6 ELISA Kit

Background:

Background: The human interferon-beta 2 gene(IFNB2) product is identical to that for the B-cell stimulation factor-2(BSF-2), the hybridoma growth factor(HGF)("interleukin-6"), and the hepatocyte stimulating factor(HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury(acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6(IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3 kDa.

Synonyms: cDNA FLJ61423, highly similar to Interleukin-6 ,

Full Gene Name: interleukin 6

Gene ID: 3569

UniProt: B4DVM1

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome