Annexin a1 antibody (N-Term)

Catalog No. ABIN2774833

Product Details anti-Annexin a1 Antibody

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Annexin a1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: WFIENEEQEY VQTVKSSKGG PGSAVSPYPT FNPSSDVAAL HKAIMVKGVD

Predicted Reactivity: Cow: 79%, Dog: 79%, Guinea Pig: 79%, Human: 100%, Mouse: 83%, Rat: 82%

Characteristics: This is a rabbit polyclonal antibody against ANXA1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ANXA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 346 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Annexin a1 antibody (ABIN2774833)

Ishida, Yamasaki, Yukizaki, Nishiyama, Tsubouchi, Okayama, Kataoka et al.: "Carnosol, rosemary ingredient, induces apoptosis in adult T-cell leukemia/lymphoma cells via glutathione depletion: proteomic approach using fluorescent two-dimensional differential gel ..." in: Human cell, Vol. 27, Issue 2, pp. 68-77, (2014) (PubMed).

Nordon, Hinchliffe, Malkawi, Pirianov, Torsney, Loftus, Cockerill, Thompson: "Comparative proteomics reveals a systemic vulnerability in the vasculature of patients with abdominal aortic aneurysms." in: Journal of vascular surgery, Vol. 54, Issue 4, pp. 1100-1108.e6, (2011) (PubMed).

Target Details for Annexin a1

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Alternative Name: ANXA1 (ANXA1 Products)

Background: ANXA1 encodes a protein that belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.
Alias Symbols: ANX1, LPC1
Protein Interaction Partner: UBC, MEIS2, SUMO2, SUMO3, WWOX, ZBTB1, HECW2, YWHAQ, ATP4A, UBD, BAG3, HMGA1, VCAM1, ITGA4, FN1, ATF2, ANXA4, RIPK1, IKBKG, RELA, CUL5, CDK2, SIRT7, ANXA5, ANXA1, KLHL23, LRIF1, OTUB1, C1orf123, CSAD, C14orf1, FAF1, COPS6, CCT7, KMT2B, UNC119, SUMO1, UBE2
Protein Size: 346

Molecular Weight: 38 kDa

Gene ID: 301

NCBI Accession: NM_000700, NP_000691

UniProt: P04083

Pathways: Hormone Transport