GluN 2B (AA 42-60) antibody

Catalog No. ABIN1742497

Product Details

Target: GluN 2B

Binding Specificity: AA 42-60

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Application: Immunoprecipitation (IP), Western Blotting (WB)

Specificity: Specific for GluN 2B

Cross-Reactivity (Details): no cross-reactivity to GluN 2A.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide (aa 42-60 of rat GluN 2B) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: not tested yet
IHC: not tested yet

Comment:

IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References

Szutorisz, DiNieri, Sweet, Egervari, Michaelides, Carter, Ren, Miller, Blitzer, Hurd: "Parental THC exposure leads to compulsive heroin-seeking and altered striatal synaptic plasticity in the subsequent generation." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 39, Issue 6, pp. 1315-23, (2014) (PubMed).

Gut, Beske, Hubbard, Lyman, Hamilton, McNutt: "Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury." in: PLoS ONE, Vol. 8, Issue 5, pp. e64423, (2013) (PubMed).

Target Details

Target: GluN 2B

Background: Synonyms: NMDA-receptor 2B