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Phospholipd Assay Kit

BCA Plasma (no EDTA), Serum
Catalog No. ABIN1000327
  • Target
    Phospholipid
    Application
    Biochemical Assay (BCA)
    Sample Type
    Serum, Plasma (no EDTA)
    Characteristics
    Sensitive. Use 20 µL samples. Linear detection range: colorimetric assay 3 - 200 µM, fluorimetric assay 0.6 - 20 µM phospholipid.
    Components
    Assay Buffer: 10 mL. PLD Enzyme: 120 µL. Enzyme Mix: 120 µL. Dye Reagent: 120 µL. Standard: 400 µL 2 mM phosphatidylcholine.
    Material not included
    Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader.
  • Application Notes
    Assays: phospholipid in biological samples such as serum and non- EDTA plasma. Drug Discovery/Pharmacology: effects of drugs on choline-containing phospholipid metabolism.
    Calculation of Results

    Subtract blank value (#4) from the standard values and plot the OD or F against standard concentrations.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
  • Dhamdhere, Fang, Jiang, Lee, Cheng, Olveda, Liu, Mulligan, Carlson, Ransom, Weis, Helms: "Drugging a stem cell compartment using Wnt3a protein as a therapeutic." in: PLoS ONE, Vol. 9, Issue 1, pp. e83650, (2014) (PubMed).

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    Kaszás, Bozó, Budai, Gróf: "Ciprofloxacin encapsulation into giant unilamellar vesicles: membrane binding and release." in: Journal of pharmaceutical sciences, Vol. 102, Issue 2, pp. 694-705, (2013) (PubMed).

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    Soga, Kinosita, Yoshida, Suzuki: "Kinetic equivalence of transmembrane pH and electrical potential differences in ATP synthesis." in: The Journal of biological chemistry, Vol. 287, Issue 12, pp. 9633-9, (2012) (PubMed).

    Haynes, Filippov, Filippova, Yang, Zhang, Duerksen-Hughes: "DNA damage induces down-regulation of UDP-glucose ceramide glucosyltransferase, increases ceramide levels and triggers apoptosis in p53-deficient cancer cells." in: Biochimica et biophysica acta, Vol. 1821, Issue 7, pp. 943-53, (2012) (PubMed).

    Mukherjee, Wu, Barbour, Fang: "Lysophosphatidic acid activates lipogenic pathways and de novo lipid synthesis in ovarian cancer cells." in: The Journal of biological chemistry, Vol. 287, Issue 30, pp. 24990-5000, (2012) (PubMed).

    Kim, Steelman, Zhang, Kinney, Li: "Aberrant upregulation of astroglial ceramide potentiates oligodendrocyte injury." in: Brain pathology (Zurich, Switzerland), Vol. 22, Issue 1, pp. 41-57, (2011) (PubMed).

    Stoll, Stadnick, Kollas, Holovati, Glasmacher, Acker, Wolkers: "Liposomes alter thermal phase behavior and composition of red blood cell membranes." in: Biochimica et biophysica acta, Vol. 1808, Issue 1, pp. 474-81, (2010) (PubMed).

  • Target
    Phospholipid
    Alternative Name
    Phospholipd
    Background
    Quantitative determination of phospholipid by colorimetric (570nm) or fluorimetric (530/585nm) methods.
    Procedure: 20 min.

    Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. This method provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.
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