CD8 antibody

Catalog No. ABIN965819

Product Details anti-CD8 Antibody

Target: CD8

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), ELISA, Immunohistochemistry (Acetone-fixed) (IHC (af))

Specificity: This antibody reacts with a 32 kD molecule present on human suppressor/cytotoxic T-cells. These antibodies also react with sinus-lining cells in the human spleen.

Purification: Purified.

Immunogen: Human peripheral lymphocytes.

Clone: B362 (RIV 11)

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
ELISA: 0.2-1.0 ug/mL.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections.

Comment:

Recommended Positive Control: Tonsil and Lymph Node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CD8 antibody (ABIN965819)

Barber, Dasgupta, Schlossman, Trevillyan, Rudd: "The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 9, pp. 3277-81, (1989) (PubMed).

Jonker, Nooij, den Butter, van Lambalgen, Fuccello: "Side effects and immunogenicity of murine lymphocyte-specific monoclonal antibodies in subhuman primates." in: Transplantation, Vol. 45, Issue 4, pp. 677-82, (1988) (PubMed).

Reinherz, Kung, Goldstein, Schlossman: "A monoclonal antibody reactive with the human cytotoxic/suppressor T cell subset previously defined by a heteroantiserum termed TH2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 124, Issue 3, pp. 1301-7, (1980) (PubMed).

Target Details for CD8

Target: CD8

Abstract: CD8 Products

Synonyms: CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody

Gene ID: 925