GR (AA 201-250), (pSer226) antibody

Catalog No. ABIN1531277

Product Details

Target: GR

Binding Specificity: AA 201-250, pSer226

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity:

GR (Phospho-Ser226) Antibody detects endogenous levels of GR only when phosphorylated at Ser226.

PhosphorylationH:S226 M:S234 R:S246

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human GR around the phosphorylation site of Ser226.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

Comment:

Unigene-Number: Hs.122926 (NCBI Gene Symbol: GCR)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References

Allan, Goggin, Caldwell: "Prenatal alcohol exposure modifies glucocorticoid receptor subcellular distribution in the medial prefrontal cortex and impairs frontal cortex-dependent learning." in: PLoS ONE, Vol. 9, Issue 4, pp. e96200, (2014) (PubMed).

Target Details

Target: GR

Background:

Synonyms: GCR, GRL, GRL1, NR3C1, glucocorticoid receptor

NCBI Gene Symbol: GCR

Molecular Weight: 85 kDa

Gene ID: 2908

OMIM: 138040

UniProt: P04150