Syntaxin 16 antibody (AA 1-302)

Catalog No. ABIN1742232

Product Details anti-Syntaxin 16 Antibody

Target: Syntaxin 16 (STX16)

Binding Specificity: AA 1-302

Reactivity: Human, Mouse, Rat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Syntaxin 16 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Recognizes all four isoforms syntaxin 16a, b, c, d.

Purification: antiserum

Immunogen: Recombinant protein of rat syntaxin 16b (aa 1-302).

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (AP staining)
ICC: 1 : 100 up to 1 : 500
IHC-P: 1 : 2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-Syntaxin 16 antibody (ABIN1742232)

Tenorio, Ross, Luchsinger, Rivera-Dictter, Arriagada, Acuña, Aguilar, Cavieres, Burgos, Ehrenfeld, Mardones: "Distinct Biochemical Pools of Golgi Phosphoprotein 3 in the Human Breast Cancer Cell Lines MCF7 and MDA-MB-231." in: PLoS ONE, Vol. 11, Issue 4, pp. e0154719, (2016) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Roccisana, Sadler, Bryant, Gould: "Sorting of GLUT4 into its insulin-sensitive store requires the Sec1/Munc18 protein mVps45." in: Molecular biology of the cell, Vol. 24, Issue 15, pp. 2389-97, (2013) (PubMed).

Pattu, Qu, Schwarz, Strauss, Weins, Bhat, Halimani, Marshall, Rettig, Hoth: "SNARE protein expression and localization in human cytotoxic T lymphocytes." in: European journal of immunology, Vol. 42, Issue 2, pp. 470-5, (2012) (PubMed).

Bogan, Rubin, Yu, Löffler, Orme, Belman, McNally, Hao, Cresswell: "Endoproteolytic cleavage of TUG protein regulates GLUT4 glucose transporter translocation." in: The Journal of biological chemistry, Vol. 287, Issue 28, pp. 23932-47, (2012) (PubMed).

Sadakata, Shinoda, Sekine, Saruta, Itakura, Takahashi, Furuichi et al.: "Interaction of calcium-dependent activator protein for secretion 1 (CAPS1) with the class II ADP-ribosylation factor small GTPases is required for dense-core vesicle trafficking in the trans-Golgi ..." in: The Journal of biological chemistry, Vol. 285, Issue 49, pp. 38710-9, (2010) (PubMed).

Nishimoto-Morita, Shin, Mitsuhashi, Kitamura, Zhang, Johannes, Nakayama: "Differential effects of depletion of ARL1 and ARFRP1 on membrane trafficking between the trans-Golgi network and endosomes." in: The Journal of biological chemistry, Vol. 284, Issue 16, pp. 10583-92, (2009) (PubMed).

Pérez-Victoria, Bonifacino: "Dual roles of the mammalian GARP complex in tethering and SNARE complex assembly at the trans-golgi network." in: Molecular and cellular biology, Vol. 29, Issue 19, pp. 5251-63, (2009) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Nakamura, Fukuda, Kato, Hirose: "MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking." in: Molecular biology of the cell, Vol. 16, Issue 4, pp. 1696-710, (2005) (PubMed).

Bose, Guilherme, Huang, Hubbard, Lane, Soriano, Czech: "The v-SNARE Vti1a regulates insulin-stimulated glucose transport and Acrp30 secretion in 3T3-L1 adipocytes." in: The Journal of biological chemistry, Vol. 280, Issue 44, pp. 36946-51, (2005) (PubMed).

Target Details for Syntaxin 16

Target: Syntaxin 16 (STX16)

Alternative Name: Syntaxin 16 (STX16 Products)

Synonyms: CG1467 antibody, Dm Syx16 antibody, DmSyx16 antibody, Dmel\\CG1467 antibody, SYX16 antibody, Syx antibody, dSyx 16 antibody, dSyx16 antibody, syntaxin-16 antibody, stx16 antibody, stx16a antibody, syn16 antibody, STX16 antibody, im:7158627 antibody, ACYPI005518 antibody, 4930401D03 antibody, 5430410K23Rik antibody, 6330500A18Rik antibody, AI648908 antibody, AW553605 antibody, Syn16 antibody, SYN16 antibody, Syntaxin 16 antibody, syntaxin 16 antibody, syntaxin 16 S homeolog antibody, Syx16 antibody, STX16 antibody, stx16.S antibody, stx16 antibody, GL50803_3869 antibody, Stx16 antibody

Pathways: Synaptic Vesicle Exocytosis