Caspase 1 (p10) antibody

Catalog No. ABIN1169386

Product Details anti-Caspase 1 (p10) Antibody

Target: Caspase 1 (p10) (CASP-1 (p10))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 1 (p10) antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Recognizes endogenous full-length and activated (p10 fragment) mouse caspase-1.

Cross-Reactivity: Mouse (Murine)

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant mouse caspase-1.

Clone: Casper-2

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-Caspase 1 (p10) antibody (ABIN1169386)

Chevriaux, Pilot, Derangère, Simonin, Martine, Chalmin, Ghiringhelli, Rébé: "Cathepsin B Is Required for NLRP3 Inflammasome Activation in Macrophages, Through NLRP3 Interaction." in: Frontiers in cell and developmental biology, Vol. 8, pp. 167, (2020) (PubMed).

Liu, Lu, Chen, Muthuraj, Li, Pattabiraman, Zempleni, Kachman, Natarajan, Yu: "Protective Role of Shiitake Mushroom-Derived Exosome-Like Nanoparticles in D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice." in: Nutrients, Vol. 12, Issue 2, (2020) (PubMed).

Deng, Guo, Tam, Johnson, Brickey, New, Lenox, Shi, Golenbock, Koller, McKinnon, Beutler, Ting: "Platelet-activating factor (PAF) mediates NLRP3-NEK7 inflammasome induction independently of PAFR." in: The Journal of experimental medicine, Vol. 216, Issue 12, pp. 2838-2853, (2020) (PubMed).

Wong, Li, Rao, Yang, Cheng, Lin, Cheng, Chen, Hua: "Repositioning of the β-Blocker Carvedilol as a Novel Autophagy Inducer That Inhibits the NLRP3 Inflammasome." in: Frontiers in immunology, Vol. 9, pp. 1920, (2018) (PubMed).

Freeman, Guo, David, Brickey, Jha, Ting: "NLR members NLRC4 and NLRP3 mediate sterile inflammasome activation in microglia and astrocytes." in: The Journal of experimental medicine, Vol. 214, Issue 5, pp. 1351-1370, (2017) (PubMed).

Yabal, Müller, Adler, Knies, Groß, Damgaard, Kanegane, Ringelhan, Kaufmann, Heikenwälder, Strasser, Groß, Ruland, Peschel, Gyrd-Hansen, Jost: "XIAP restricts TNF- and RIP3-dependent cell death and inflammasome activation." in: Cell reports, Vol. 7, Issue 6, pp. 1796-808, (2014) (PubMed).

Target Details for Caspase 1 (p10)

Target: Caspase 1 (p10) (CASP-1 (p10))

Alternative Name: Caspase-1 p10 (CASP-1 (p10) Products)

Background: Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.

UniProt: P29452