Histone 3 antibody (H3K36me2)

Catalog No. ABIN4889654

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K36me2

Reactivity: Human, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Purification: None

Immunogen: This Histone H3 dimethylLys36 antibody was raised against a peptide including dimethyllysine 36 of histone H3.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Histone 3 antibody (ABIN4889654)

Young, Hillyer, Hokamp, Fitzpatrick, Konstantinov, Welty, Ness, Werner-Washburne, Fleming, Osley: "Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast." in: BMC genomics, Vol. 18, Issue 1, pp. 107, (2017) (PubMed).

García-Carpizo, Sarmentero, Han, Graña, Ruiz-Llorente, Pisano, Serrano, Brooks, Campbell, Barrero: "NSD2 contributes to oncogenic RAS-driven transcription in lung cancer cells through long-range epigenetic activation." in: Scientific reports, Vol. 6, pp. 32952, (2016) (PubMed).

Luense, Denner, Fernández-Montalván, Hartung, Husemann, Stresemann, Prechtl: "Quantification of histone H3 Lys27 trimethylation (H3K27me3) by high-throughput microscopy enables cellular large-scale screening for small-molecule EZH2 inhibitors." in: Journal of biomolecular screening, Vol. 20, Issue 2, pp. 190-201, (2015) (PubMed).

Feng, Zhang, Shea, Creighton, Coarfa, Hilsenbeck, Lanz, He, Wang, Fu, Nardone, Song, Bradner, Mitsiades, Mitsiades, Osborne, Schiff, OMalley: "An epigenomic approach to therapy for tamoxifen-resistant breast cancer." in: Cell research, Vol. 24, Issue 7, pp. 809-19, (2014) (PubMed).

Jha, Strahl: "An RNA polymerase II-coupled function for histone H3K36 methylation in checkpoint activation and DSB repair." in: Nature communications, Vol. 5, pp. 3965, (2014) (PubMed).

Ma, Pan, Montgomery, Olson, Schultz: "Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 8, pp. E481-9, (2012) (PubMed).

Hainer, Martens: "Identification of histone mutants that are defective for transcription-coupled nucleosome occupancy." in: Molecular and cellular biology, Vol. 31, Issue 17, pp. 3557-68, (2011) (PubMed).

Hsia, Tepper, Pochampalli, Hsia, Izumiya, Huerta, Wright, Chen, Kung, Izumiya: "KDM8, a H3K36me2 histone demethylase that acts in the cyclin A1 coding region to regulate cancer cell proliferation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 21, pp. 9671-6, (2010) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Molecular Weight: 17 kDa

Gene ID: 3020