PsbE antibody

Catalog No. ABIN6254845

Product Details

Target: PsbE

Reactivity: Arabidopsis thaliana, Barley, Nicotiana tabacum, Spinach

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Cross-Reactivity (Details): Not reactive in: Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942

Predicted Reactivity: dicots including Glycine max, Salvia miltiorrhiza, Solanum tuberosum, deciduous flowering plant Populus alba

Characteristics: Expected / apparent Molecular Weight of the Antigene: 9.25 kDa

Purification: serum

Immunogen: KLH-conjugated synthetic peptide chosen from PsbE protein of Arabidopsis thaliana P56779, AtCg00580

Application Details

Application Notes: 1 : 5000 with standard ECL (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References

Ido, Nield, Fukao, Nishimura, Sato, Ifuku: "Cross-linking evidence for multiple interactions of the PsbP and PsbQ proteins in a higher plant photosystem II supercomplex." in: The Journal of biological chemistry, Vol. 289, Issue 29, pp. 20150-7, (2014) (PubMed).

Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).

Shang, Yan, Liu, Cao, Mei, Xin, Wu, Wang, Du, Jiang, Zhang, Zhao, Sun, Liu, Yu, Zhang: "The Mg-Chelatase H Subunit of Arabidopsis Antagonizes a Group of Transcription Repressors to Relieve ABA-Responsive Genes of Inhibition." in: The Plant cell, (2010) (PubMed).

Target Details

Target: PsbE

Background: AGI Code: ATCG00580
Cytochrome b559 (Cyt b559) is encoded by the chloroplast genes psbE and psbF and is comprised of two low molecular mass polypeptides, a and h subunits, with molecular masses of 9 and 4 kDa, respectively. The Cyt b559 is closely associated with PSII in all oxygenic photosynthetic organisms. The a and h subunits of the Cyt b559 are components of the minimal PSII reaction center complex that is still capable of primary charge separation In summary, both PsbE and PsbF are essential components for PSII assembly, and they are probably involved in electron transport mechanisms that help to protect PSII from photodamage.Altrnative protein name: PSII reaction center subunit V

Molecular Weight: 9.25 kDa

UniProt: P56779