NF-kappa B (NF-kB) Activation Assay Kit

Catalog No. ABIN1110858

Product Details

Target: NFkB (Nuclear Factor kappa B (NFkB))

Reactivity: Human

Application: Activity Assay (AcA), Western Blotting (WB)

Purpose: Rapid assay to quantitate NF-kappa B activation using cell fractionation into nuclear and cytoplasmic components

Specificity: This NF-KB activation assay follows the translocation of the p65 (RelA) subunit of NF-kB into the nucleus, a hallmark of NF-kappa B activation. Cytoplasmic and nuclear fractions are isolated, and Western blots of the fractions are used to quantify the relative p65 concentration. The ratio of nuclear relative to cytoplasmic p65 concentration provides a numerical estimate of NF-kappa B activation.

Characteristics: This NF-KB Activation Assay Kit provides all the reagents required to fractionate cells into cytoplasmic and nuclear fractions, as well as a selective and high affinity antibody to detect p65 in Western blots.

Components:

• Cytoplasmic Fractionation Reagent (CER-1, 55mL)
• Nuclear Fractionation Reagent (NER-1, 3.5 mL)
• DTT (sufficient to make a 1M solution, volume 64 microliters)
• Rabbit Polyclonal IgG Antibody to p65. (100 µL Use at 1:100 - 1:400 dilution for Western blots)

Application Details

Application Notes: Use at 1:400 dilution for Western blots

Comment:

Cytoplasmic Fractionation Reagent (55 mL)
Nuclear Fractionation Reagent (3.5 mL)
DTT (sufficient to make a 1 M solution, volume 64 µL)

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: p65Ab: Aliquot, -20°C.
HRPAb: 4°C.
Other components: -20°C

References

Shi, Luo, Wang, Long: "Incorporation of β-sitosterol into the membrane prevents tumor necrosis factor-α-induced nuclear factor-κB activation and gonadotropin-releasing hormone decline." in: Steroids, Vol. 96, pp. 1-6, (2015) (PubMed).

Dattaroy, Pourhoseini, Das, Alhasson, Seth, Nagarkatti, Michelotti, Diehl, Chatterjee: "Micro-RNA 21 inhibition of SMAD7 enhances fibrogenesis via leptin-mediated NADPH oxidase in experimental and human nonalcoholic steatohepatitis." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 308, Issue 4, pp. G298-312, (2015) (PubMed).

Zou, Ding, Zhan, Liu, Pan, Xia: "Glyoxalase I is differentially expressed in cutaneous neoplasms and contributes to the progression of squamous cell carcinoma." in: The Journal of investigative dermatology, Vol. 135, Issue 2, pp. 589-98, (2015) (PubMed).

Martinotti, Ranzato, Parodi, Vitale, Burlando: "Combination of ascorbate/epigallocatechin-3-gallate/gemcitabine synergistically induces cell cycle deregulation and apoptosis in mesothelioma cells." in: Toxicology and applied pharmacology, Vol. 274, Issue 1, pp. 35-41, (2013) (PubMed).

Target Details

Target: NFkB (Nuclear Factor kappa B (NFkB))

Alternative Name: NF-kappa B (NFkB Products)

Synonyms: EBP-1 Kit, KBF1 Kit, NF-kB1 Kit, NF-kappa-B Kit, NF-kappaB Kit, NFKB-p105 Kit, NFKB-p50 Kit, NFkappaB Kit, p105 Kit, p50 Kit, NF-kB Kit, NF-KB1 Kit, NF-kappaB1 Kit, p50/p105 Kit, nuclear factor kappa B subunit 1 Kit, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 Kit, NFKB1 Kit, Nfkb1 Kit