Histone 3 antibody (N-Term)

Catalog No. ABIN2668390

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC)

Purification: Protein G Chromatography

Immunogen: This Histone H3 antibody (mAb) was raised against a peptide containing the N-terminus of histone H3.

Clone: MABI 0301

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.54 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Histone 3 antibody (ABIN2668390)

Bulusu, Tumanov, Michalopoulou, van den Broek, MacKay, Nixon, Dhayade, Schug, Vande Voorde, Blyth, Gottlieb, Vazquez, Kamphorst: "Acetate Recapturing by Nuclear Acetyl-CoA Synthetase 2 Prevents Loss of Histone Acetylation during Oxygen and Serum Limitation." in: Cell reports, Vol. 18, Issue 3, pp. 647-658, (2017) (PubMed).

Pacaud, Cheray, Nadaradjane, Vallette, Cartron: "Histone H3 phosphorylation in GBM: a new rational to guide the use of kinase inhibitors in anti-GBM therapy." in: Theranostics, Vol. 5, Issue 1, pp. 12-22, (2015) (PubMed).

Wagner, Ciszewski, Kania: "L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation." in: Cell communication and signaling : CCS, Vol. 13, pp. 36, (2015) (PubMed).

Zhan, Kost-Alimova, Shi, Leo, Bardenhagen, Shepard, Appikonda, Vangamudi, Zhao, Tieu, Jiang, Heffernan, Marszalek, Toniatti, Draetta, Tyler, Barton, Jones, Palmer, Geck Do, Andersen: "Development of novel cellular histone-binding and chromatin-displacement assays for bromodomain drug discovery." in: Epigenetics & chromatin, Vol. 8, pp. 37, (2015) (PubMed).

Fukuda, Mitani, Miyashita, Umezawa, Akutsu: "Chromatin condensation of Xist genomic loci during oogenesis in mice." in: Development (Cambridge, England), Vol. 142, Issue 23, pp. 4049-55, (2015) (PubMed).

van de Werken, van der Heijden, Eleveld, Teeuwssen, Albert, Baarends, Laven, Peters, Baart: "Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications." in: Nature communications, Vol. 5, pp. 5868, (2014) (PubMed).

Murphy, Cipriany, Wallin, Ju, Szeto, Hagarman, Benitez, Craighead, Soloway: "Single-molecule analysis of combinatorial epigenomic states in normal and tumor cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 19, pp. 7772-7, (2013) (PubMed).

Ohmori, Takai, Ishijima, Suzuki, Moriguchi, Philipsen, Yamamoto, Ohneda: "Regulation of GATA factor expression is distinct between erythroid and mast cell lineages." in: Molecular and cellular biology, Vol. 32, Issue 23, pp. 4742-55, (2012) (PubMed).

Cerf, Tian, Craighead: "Ordered arrays of native chromatin molecules for high-resolution imaging and analysis." in: ACS nano, Vol. 6, Issue 9, pp. 7928-34, (2012) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Molecular Weight: 17 kDa

Gene ID: 3020