Avi-Tag antibody

Catalog No. ABIN1573913

Product Details anti-Avi-Tag Antibody

Target: Avi-Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Avi-Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Sequence: GLNDIFEAQKIEWHE

Specificity: This Antibody recognizes C-terminal, N-terminal, and internal Avi tagged fusion proteins.

Purification: Immunoaffinity chromatography

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-Avi-Tag antibody (ABIN1573913)

Nogal, McCoy, van Gils, Cottrell, Voss, Andrabi, Pauthner, Liang, Messmer, Nedellec, Shin, Turner, Ozorowski, Sanders, Burton, Ward: "HIV envelope trimer-elicited autologous neutralizing antibodies bind a region overlapping the N332 glycan supersite." in: Science advances, Vol. 6, Issue 23, pp. eaba0512, (2020) (PubMed).

Sheridan, Fong, DAlessandro, Bentley: "Widespread Backtracking by RNA Pol II Is a Major Effector of Gene Activation, 5' Pause Release, Termination, and Transcription Elongation Rate." in: Molecular cell, Vol. 73, Issue 1, pp. 107-118.e4, (2019) (PubMed).

Hill, Martinko, Nguyen, Wells: "Human antibody-based chemically induced dimerizers for cell therapeutic applications." in: Nature chemical biology, Vol. 14, Issue 2, pp. 112-117, (2019) (PubMed).

Verdurmen, Luginbühl, Honegger, Plückthun: "Efficient cell-specific uptake of binding proteins into the cytoplasm through engineered modular transport systems." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 200, pp. 13-22, (2015) (PubMed).

Neef, Koedijk, Bosma, van Dijl, Buist: "Efficient production of secreted staphylococcal antigens in a non-lysing and proteolytically reduced Lactococcus lactis strain." in: Applied microbiology and biotechnology, Vol. 98, Issue 24, pp. 10131-41, (2014) (PubMed).

Iovino, Orihuela, Moorlag, Molema, Bijlsma: "Interactions between blood-borne Streptococcus pneumoniae and the blood-brain barrier preceding meningitis." in: PLoS ONE, Vol. 8, Issue 7, pp. e68408, (2014) (PubMed).

Lam, Cho, Lesch, Gosselin, Heinz, Tanaka-Oishi, Benner, Kaikkonen, Kim, Kosaka, Lee, Watt, Grossman, Rosenfeld, Evans, Glass: "Rev-Erbs repress macrophage gene expression by inhibiting enhancer-directed transcription." in: Nature, Vol. 498, Issue 7455, pp. 511-5, (2013) (PubMed).

Stender, Pascual, Liu, Kaikkonen, Do, Spann, Boutros, Perrimon, Rosenfeld, Glass: "Control of proinflammatory gene programs by regulated trimethylation and demethylation of histone H4K20." in: Molecular cell, Vol. 48, Issue 1, pp. 28-38, (2012) (PubMed).

Sung, Maloney, Yang, Wu: "A novel method for producing mono-biotinylated, biologically active neurotrophic factors: an essential reagent for single molecule study of axonal transport." in: Journal of neuroscience methods, Vol. 200, Issue 2, pp. 121-8, (2011) (PubMed).

Janes, Griesshaber, Atapattu, Nievergall, Hii, Mensinga, Chheang, Day, Boyd, Bastiaens, Jørgensen, Pawson, Lackmann: "Eph receptor function is modulated by heterooligomerization of A and B type Eph receptors." in: The Journal of cell biology, Vol. 195, Issue 6, pp. 1033-45, (2011) (PubMed).

Target Details for Avi-Tag

Target: Avi-Tag

Abstract: Avi-Tag Products

Target Type: Tag

Background: Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems. The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Rabbit Anti-Avi-tag Polyclonal Antibody is developed in rabbit using a synthetic Avi tag peptide conjugated to KLH.