CD11a (Activitor) antibody
Reactivity: Human
FACS, IP
Host: Mouse
Monoclonal
NKI(SPV)-L16
unconjugated
(2 references)-
- Target
- CD11a (Activitor)
- Reactivity
- Human
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Flow Cytometry (FACS), Immunoprecipitation (IP)
- Specificity
- This antibody recognizes an activation epitope of CD11a and has been essential to define inside-out signaling of intergrin. Homotypic cell aggregate formation is stimulated (see also Background).
- Characteristics
- CD markers,Cell adhesion
- Purification
- Purified
- Clone
- NKI(SPV)-L16
- Isotype
- IgG2a
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- Application Notes
- NKI(SPV)-L16 is useful for flow cytometry and, immunoprecipitation. Optimal antibody dilution should be determined by titration.
- Comment
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The antibody was raised by immunization of Balb/c mice with monocytes. The unique activation dependent epitope of the SPV-L16 antibody on the a-chain of LFA-1 is relatively insensitive to trypsin treatment and thus most likely located close to the transmembrane segment of the protein.
- Restrictions
- For Research Use only
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- Buffer
- PBS containing 0.09% sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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: "Enhancement of LFA-1-mediated cell adhesion by triggering through CD2 or CD3 on T lymphocytes." in: Nature, Vol. 342, Issue 6251, pp. 811-3, (1990) (PubMed).
: "A monoclonal antibody (NKI-L16) directed against a unique epitope on the alpha-chain of human leukocyte function-associated antigen 1 induces homotypic cell-cell interactions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 5, pp. 1393-400, (1988) (PubMed).
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: "Enhancement of LFA-1-mediated cell adhesion by triggering through CD2 or CD3 on T lymphocytes." in: Nature, Vol. 342, Issue 6251, pp. 811-3, (1990) (PubMed).
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- Target
- CD11a (Activitor)
- Background
- Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide), also known as ITGAL, is a human gene which functions in the immune system. It is involved in cellular adhesion and costimulatory signaling. ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 is part of the family of leukocyte integrins that are recognised by their common beta-chains (CD18). LFA-1 also has a distinct alpha-chain (CD11a). LFA-1 is expressed on lymphocytes, monocytes and granulocytes, it has been shown that LFA-1 contributes to the adhesion reaction of these cells. Blocking experiments with anti-LFA monoclonal antibodies demonstrated that LFA-1 inhibits the adhesion step effector and target cells in cytotoxic T lymphocyte, natural killer and lectin dependent cytotoxicity. LFA-1 is involved in recruitment to the site of infection. It binds to ICAM-1 on antigen-presenting cells and functions as an adhesion molecule. LFA-1 is the first to bind T-cells to antigen-presenting cells and initially binds weakly. A signal from the T-cell receptor and/or the cytokine receptor changes the conformation and prolongs the cell contact, allowing the T-cell to proliferate. A unique antibody NKI(SPV)-L16, reacting with the a-chain of the human leukocyte function-associated Ag- 1 (LFA- 1) has been made available. This antibody stimulates homotypic cell-cell interactions in a manner very similar to 12-0-tetradecanoyl-phorbol1-3 -acetate (TPA), in contrast to other anti-LFA-1 mAb which inhibit cell aggregation. Immunoprecipitation and enzyme digestion studies revealed that NKI-L16 recognizes a unique epitope on the cy-chain of LFA-1,most likely situated close to the transmembrane segment of the molecule. It is hypothesized that NKI-L16 or TPA can cause the LFA-1 molecule to convert from an inactive to an active configuration, thereby permitting binding of LFA-1 1 to its natural ligand. This mAb directed, in contrast to other anti-LFA-1 antibodies, stimulates rather than inhibits homotypic cell aggregate formation, but does not affect cell proliferation.
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