Histone 3 antibody (H3K4me3)

Catalog No. ABIN2668474

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K4me3

Reactivity: Human, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Purification: Protein A Chromatography

Immunogen: This Histone H3 trimethylLys4 antibody was raised against a peptide including trimethyllysine 4 of histone H3.

Isotype: IgG

Application Details

Application Notes: Recommended starting concentrations are
ChIP: 3 µg per ChIP
ChIP-Seq: 4 µg each
ICC/IF: 1 - 2 µg/mL dilution
WB: 2 - 4 µg/mL dilution
CUT&RUN: 1:100
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Histone 3 antibody (ABIN2668474)

Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).

Akiyama, Koda, Byeon, Shimada, Nishikawaji, Sakamoto, Chen, Kojima, Kawano, Eishi, Deng, Kim, Zhu, Yuasa, Tanaka: "Reduced expression of SET7/9, a histone mono-methyltransferase, is associated with gastric cancer progression." in: Oncotarget, Vol. 7, Issue 4, pp. 3966-83, (2016) (PubMed).

Singh, Johnstone, Martin, Tempera, Kaplan, Denny: "Alterations in nuclear structure promote lupus autoimmunity in a mouse model." in: Disease models & mechanisms, Vol. 9, Issue 8, pp. 885-97, (2016) (PubMed).

Heubach, Monsior, Deenen, Niegisch, Szarvas, Niedworok, Schulz, Hoffmann: "The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cells." in: Molecular cancer, Vol. 14, pp. 108, (2015) (PubMed).

Cabrera, Olcese, Horabin: "A balancing act: heterochromatin protein 1a and the Polycomb group coordinate their levels to silence chromatin in Drosophila." in: Epigenetics & chromatin, Vol. 8, pp. 17, (2015) (PubMed).

Sakashita, Kawabata, Jincho, Tajima, Kumamoto, Kobayashi, Matsui, Kono: "Sex Specification and Heterogeneity of Primordial Germ Cells in Mice." in: PLoS ONE, Vol. 10, Issue 12, pp. e0144836, (2015) (PubMed).

Walter, Matter, Fahrenkrog: "Loss of histone H3 methylation at lysine 4 triggers apoptosis in Saccharomyces cerevisiae." in: PLoS genetics, Vol. 10, Issue 1, pp. e1004095, (2014) (PubMed).

DesRochers, Shamis, Alt-Holland, Kudo, Takata, Wang, Jackson-Grusby, Garlick: "The 3D tissue microenvironment modulates DNA methylation and E-cadherin expression in squamous cell carcinoma." in: Epigenetics, Vol. 7, Issue 1, pp. 34-46, (2012) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Molecular Weight: 17 kDa

Gene ID: 3020