AMBN Protein (AA 1-447) (GST tag)
(1 reference)-
- Target See all AMBN Proteins
- AMBN (Ameloblastin (Enamel Matrix Protein) (AMBN))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-447
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This AMBN protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Antibody Array (AA), Affinity Purification (AP)
- Purpose
- AMBN (Human) Recombinant Protein (P01)
- Sequence
- MSASKIPLFK MKDLILILCL LEMSFAVPFF PQQSGTPGMA SLSLETMRQL GSLQRLNTLS QYSRYGFGKS FNSLWMHGLL PPHSSLPWMR PREHETQQYE YSLPVHPPPL PSQPSLKPQQ PGLKPFLQSA AATTNQATAL KEALQPPIHL GHLPLQEGEL PLVQQQVAPS DKPPKPELPG VDFADPQGPS LPGMDFPDPQ GPSLPGLDFA DPQGSTIFQI ARLISHGPMP QNKQSPLYPG MLYVPFGANQ LNAPARLGIM SSEEVAGGRE DPMAYGAMFP GFGGMRPGFE GMPHNPAMGG DFTLEFDSPV AATKGPENEE GGAQGSPMPE ANPDNLENPA FLTELEPAPH AGLLALPKDD IPGLPRSPSG KMKGLPSVTP AAADPLMTPE LADVYRTYDA DMTTSVDFQE EATMDTTMAP NSLQTSMPGN KAQEPEMMHD AWHFQEP
- Characteristics
- Human AMBN full-length ORF ( NP_057603.1, 1 a.a. - 447 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Ameloblastin (Enamel Matrix Protein) (AMBN) (AA 27-447) protein (His tag)
Crystallography grade AMBN Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Ameloblastin (Enamel Matrix Protein) (AMBN) (AA 27-407) protein (His tag)Crystallography grade AMBN Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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-
: "Protein Interaction between Ameloblastin and Proteasome Subunit ? Type 3 Can Facilitate Redistribution of Ameloblastin Domains within Forming Enamel." in: The Journal of biological chemistry, Vol. 290, Issue 34, pp. 20661-73, (2015) (PubMed).
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: "Protein Interaction between Ameloblastin and Proteasome Subunit ? Type 3 Can Facilitate Redistribution of Ameloblastin Domains within Forming Enamel." in: The Journal of biological chemistry, Vol. 290, Issue 34, pp. 20661-73, (2015) (PubMed).
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- Target
- AMBN (Ameloblastin (Enamel Matrix Protein) (AMBN))
- Alternative Name
- AMBN (AMBN Products)
- Synonyms
- ameloblastin Protein, Ambn Protein, AMBN Protein
- Background
-
Full Gene Name: ameloblastin (enamel matrix protein)
Synonyms: - Gene ID
- 258
- NCBI Accession
- NM_016519
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