GABARAP antibody (AA 1-30)

Catalog No. ABIN388564

Product Details anti-GABARAP Antibody

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Binding Specificity: AA 1-30

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABARAP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: B, Rb

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This GABARAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids of human GABARAP.

Clone: RB11846

Isotype: IgG

Application Details

Application Notes: IF: 1:25. IF: 1:25. WB: 1:1000-1 :2000. IHC-P: 1:25. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-GABARAP antibody (ABIN388564)

Joachim, Razi, Judith, Wirth, Calamita, Encheva, Dynlacht, Snijders, OReilly, Jefferies, Tooze: "Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy." in: Current biology : CB, Vol. 27, Issue 14, pp. 2123-2136.e7, (2018) (PubMed).

Clough, Wright, Pereira, Hirst, Johnston, Henriques, Frickel: "K63-Linked Ubiquitination Targets Toxoplasma gondii for Endo-lysosomal Destruction in IFNγ-Stimulated Human Cells." in: PLoS pathogens, Vol. 12, Issue 11, pp. e1006027, (2017) (PubMed).

Egan, Chun, Vamos, Zou, Rong, Miller, Lou, Raveendra-Panickar, Yang, Sheffler, Teriete, Asara, Turk, Cosford, Shaw: "Small Molecule Inhibition of the Autophagy Kinase ULK1 and Identification of ULK1 Substrates." in: Molecular cell, Vol. 59, Issue 2, pp. 285-97, (2015) (PubMed).

Orfali, ODonovan, Nyhan, Britschgi, Tschan, Cahill, Mongan, Gudas, McKenna: "Induction of autophagy is a key component of all-trans-retinoic acid-induced differentiation in leukemia cells and a potential target for pharmacologic modulation." in: Experimental hematology, Vol. 43, Issue 9, pp. 781-93.e2, (2015) (PubMed).

Target Details for GABARAP

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Alternative Name: GABARAP (GABARAP Products)

Background: Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABARAP is GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.

Molecular Weight: 13918

Gene ID: 11337

NCBI Accession: NP_009209

UniProt: O95166

Pathways: Autophagy