WNT3 Protein (AA 1-355) (GST tag)
(1 reference)-
- Target See all WNT3 Proteins
- WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-355
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This WNT3 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- WNT3 (Human) Recombinant Protein (P01)
- Sequence
- MEPHLLGLLL GLLLGGTRVL AGYPIWWSLA LGQQYTSLGS QPLLCGSIPG LVPKQLRFCR NYIEIMPSVA EGVKLGIQEC QHQFRGRRWN CTTIDDSLAI FGPVLDKATR ESAFVHAIAS AGVAFAVTRS CAEGTSTICG CDSHHKGPPG EGWKWGGCSE DADFGVLVSR EFADARENRP DARSAMNKHN NEAGRTTILD HMHLKCKCHG LSGSCEVKTC WWAQPDFRAI GDFLKDKYDS ASEMVVEKHR ESRGWVETLR AKYSLFKPPT ERDLVYYENS PNFCEPNPET GSFGTRDRTC NVTSHGIDGC DLLCCGRGHN TRTEKRKEKC HCIFHWCCYV SCQECIRIYD VHTCK
- Characteristics
- Human WNT3 full-length ORF ( NP_110380.1, 1 a.a. - 355 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (AA 22-355) protein (His tag)
Crystallography grade WNT3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (AA 22-355) protein (His tag)WNT3 Origin: Mouse Host: Yeast Recombinant > 90 % SDS
Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (AA 22-355) protein (His-SUMO Tag)WNT3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Thalamic WNT3 Secretion Spatiotemporally Regulates the Neocortical Ribosome Signature and mRNA Translation to Specify Neocortical Cell Subtypes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 31, pp. 10911-26, (2015) (PubMed).
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: "Thalamic WNT3 Secretion Spatiotemporally Regulates the Neocortical Ribosome Signature and mRNA Translation to Specify Neocortical Cell Subtypes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 31, pp. 10911-26, (2015) (PubMed).
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- Target
- WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))
- Alternative Name
- WNT3 (WNT3 Products)
- Synonyms
- INT4 Protein, Ci-Wnt3 Protein, WNT3 Protein, int4 Protein, Xwnt3 Protein, wnt-3 Protein, wnt3l Protein, Xwnt-3 Protein, wnt3 Protein, Int-4 Protein, Wnt-3 Protein, wnt3-A Protein, Wnt family member 3 Protein, wingless-type MMTV integration site family, member 3 Protein, proto-oncogene Wnt-3 Protein, wingless-type MMTV integration site family member 3 Protein, Wnt family member 3A Protein, wingless-type MMTV integration site family member 3 L homeolog Protein, WNT3 Protein, Wnt3 Protein, wnt3 Protein, LOC468297 Protein, WNT3A Protein, wnt3.L Protein
- Background
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Full Gene Name: wingless-type MMTV integration site family, member 3
Synonyms: INT4,MGC131950,MGC138321,MGC138323 - Gene ID
- 7473
- NCBI Accession
- NM_030753
- Pathways
- WNT Signaling, Regulation of Cell Size
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