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CD45 antibody (FITC)

PTPRC Reactivity: Human, Mouse, Cat FACS Host: Rat Monoclonal RA3-6B2 FITC
Catalog No. ABIN1774735
  • Target See all CD45 (PTPRC) Antibodies
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    Reactivity
    • 393
    • 226
    • 79
    • 29
    • 20
    • 15
    • 15
    • 10
    • 7
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Cat
    Host
    • 282
    • 177
    • 148
    • 2
    • 1
    • 1
    Rat
    Clonality
    • 466
    • 137
    • 1
    Monoclonal
    Conjugate
    • 175
    • 59
    • 51
    • 48
    • 39
    • 15
    • 13
    • 11
    • 10
    • 10
    • 9
    • 9
    • 8
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD45 antibody is conjugated to FITC
    Application
    • 502
    • 207
    • 152
    • 127
    • 126
    • 74
    • 68
    • 58
    • 47
    • 27
    • 17
    • 15
    • 14
    • 13
    • 12
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    The rat monoclonal antibody RA3-6B2 recognizes an extracellular epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.
    Cross-Reactivity (Details)
    Human, Mouse, Feline (Cat)
    Purification
    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    Abelson murine leukemia virus-induced pre-B tumor cells
    Clone
    RA3-6B2
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product PTPRC Primary Antibody
  • Application Notes
    Flow cytometry: Recommended dilution: 1-4 μg/mL.
    Comment

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Lochhead, Sonderegger, Ma, Brewster, Cornwall, Maylor-Hagen, Miller, Zachary, Weis, Weis: "Endothelial cells and fibroblasts amplify the arthritogenic type I IFN response in murine Lyme disease and are major sources of chemokines in Borrelia burgdorferi-infected joint tissue." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 5, pp. 2488-501, (2012) (PubMed).

    Montecino-Rodriguez, Leathers, Dorshkind: "Identification of a B-1 B cell-specified progenitor." in: Nature immunology, Vol. 7, Issue 3, pp. 293-301, (2006) (PubMed).

    Rothaeusler, Baumgarth: "Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 69, Issue 4, pp. 249-59, (2006) (PubMed).

    Cappione, Pugh-Bernard, Anolik, Sanz: "Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 7, pp. 4298-307, (2004) (PubMed).

    Tsurui, Nishimura, Hattori, Hirose, Okumura, Shirai: "Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 48, Issue 5, pp. 653-62, (2000) (PubMed).

    Rada, Ehrenstein, Neuberger, Milstein: "Hot spot focusing of somatic hypermutation in MSH2-deficient mice suggests two stages of mutational targeting." in: Immunity, Vol. 9, Issue 1, pp. 135-41, (1998) (PubMed).

    Watanabe, Akaike: "Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells." in: Scandinavian journal of immunology, Vol. 39, Issue 5, pp. 419-25, (1994) (PubMed).

  • Target
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    Alternative Name
    CD45R (PTPRC Products)
    Synonyms
    PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody
    Background
    CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.,PTPRCR, T200R
    Pathways
    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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