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CD86 antibody

CD86 Reactivity: Human FACS, WB, IP, IHC (fro) Host: Mouse Monoclonal BU63 unconjugated
Catalog No. ABIN302084
  • Target See all CD86 Antibodies
    CD86
    Reactivity
    • 138
    • 116
    • 50
    • 20
    • 10
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    Human
    Host
    • 79
    • 68
    • 67
    Mouse
    Clonality
    • 151
    • 60
    • 1
    Monoclonal
    Conjugate
    • 85
    • 23
    • 18
    • 14
    • 13
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD86 antibody is un-conjugated
    Application
    • 144
    • 96
    • 43
    • 41
    • 33
    • 31
    • 29
    • 23
    • 16
    • 16
    • 15
    • 14
    • 13
    • 11
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    The mouse monoclonal antibody BU63 reacts with an extracellular epitope of CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
    Cross-Reactivity (Details)
    Human, Other not determined
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    B-lymphoblastoid cell line ARH 77
    Clone
    BU63
    Isotype
    IgG1
    Top Product
    Discover our top product CD86 Primary Antibody
  • Application Notes
    Flow cytometry: Recommended dilution: 5 μg/mL.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, (2011) (PubMed).

    Kolar, Mehta, Pelayo, Capra: "A novel human B cell subpopulation representing the initial germinal center population to express AID." in: Blood, Vol. 109, Issue 6, pp. 2545-52, (2007) (PubMed).

    Chan, Baird, Mercer, Fleming: "Maturation and function of human dendritic cells are inhibited by orf virus-encoded interleukin-10." in: The Journal of general virology, Vol. 87, Issue Pt 11, pp. 3177-81, (2006) (PubMed).

    Zhan, Towler, Calder: "The immunomodulatory role of human conjunctival epithelial cells." in: Investigative ophthalmology & visual science, Vol. 44, Issue 9, pp. 3906-10, (2003) (PubMed).

    Mauri, Wyss-Coray, Gallati, Pichler: "Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 118-27, (1995) (PubMed).

    Caux, Vanbervliet, Massacrier, Azuma, Okumura, Lanier, Banchereau: "B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1841-7, (1994) (PubMed).

    Engel, Gribben, Freeman, Zhou, Nozawa, Abe, Nadler, Wakasa, Tedder: "The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen." in: Blood, Vol. 84, Issue 5, pp. 1402-7, (1994) (PubMed).

  • Target
    CD86
    Alternative Name
    CD86 (CD86 Products)
    Synonyms
    B7-2 antibody, B7.2 antibody, B70 antibody, CD28LG2 antibody, LAB72 antibody, B7 antibody, CLS1 antibody, Cd28l2 antibody, ETC-1 antibody, Ly-58 antibody, Ly58 antibody, MB7 antibody, MB7-2 antibody, TS/A-2 antibody, CD86 molecule antibody, CD86 antigen antibody, integrin subunit alpha 2b antibody, CD86 antibody, Cd86 antibody, Itga2b antibody
    Background
    CD86 Molecule,CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 Molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet, there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.,B7-2, FUN-1, LAB72
    Gene ID
    942
    UniProt
    P42081
    Pathways
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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