SNAP alpha/beta antibody

Catalog No. ABIN1742242

Product Details anti-SNAP alpha/beta Antibody

Target: SNAP alpha/beta

Reactivity: Human, Rat, Zebrafish (Danio rerio), Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SNAP alpha/beta antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificity: Specific for alpha- and beta SNAP

Cross-Reactivity (Details): does not cross-react to gamma SNAP.

Purification: purified IgG

Immunogen: Recombinant full length rat alpha SNAP.

Clone: 77-2

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 10000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC: not tested yet

Comment:

The antibody does not immunoprecipitate the 20 S SNARE-complex.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-SNAP alpha/beta antibody (ABIN1742242)

de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Hanson, Otto, Barton, Jahn: "The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin." in: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16955-61, (1995) (PubMed).

Target Details for SNAP alpha/beta

Target: SNAP alpha/beta

Abstract: SNAP alpha/beta Products