CD20 antibody (APC)
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- Target See all CD20 (MS4A1) Antibodies
- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD20 antibody is conjugated to APC
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Cross-Reactivity (Details)
- Cross reactivity with CD20 from other sources has not been tested
- Purification
- Allophycocyanin conjugated CD20 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD20. Reactivity is observed against human CD20 Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, and Squirrel Monkey. . Anti-CD20 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.
- Immunogen
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Immunogen: Anti-CD20 Antibody (Monoclonal) was produced by repeated immunizations with CD20 antigen.
- Clone
- 2H7
- Isotype
- IgG2b
- Labeling Ratio
- 1-2
- Top Product
- Discover our top product MS4A1 Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-CD20 is useful for Flow Cytometry, Immunoprecipitation, and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.
Immunoprecipitation Dilution: User Optimized
Flow Cytometry Dilution: 5 µL/1x10e6 cells or 100μL of whole blood
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.2 % BSA (w/v)
0.09 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
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- Target
- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
- Alternative Name
- CD20 (MS4A1 Products)
- Synonyms
- B1 antibody, Bp35 antibody, CD20 antibody, CVID5 antibody, LEU-16 antibody, MS4A2 antibody, S7 antibody, AA960661 antibody, Cd20 antibody, Ly-44 antibody, Ms4a2 antibody, MS4A1 antibody, bp35 antibody, cd20 antibody, ms4a2 antibody, leu-16 antibody, ms4a4c antibody, cd20-like antibody, membrane spanning 4-domains A1 antibody, membrane-spanning 4-domains, subfamily A, member 1 antibody, B-lymphocyte antigen CD20 antibody, MS4A1 antibody, Ms4a1 antibody, ms4a1 antibody, LOC101694281 antibody
- Background
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Synonyms: B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20
Background: CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 Molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.
Gene Name: MS4A1
- Gene ID
- 931
- NCBI Accession
- NP_068769
- UniProt
- P11836
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