Nitrotyrosine antibody (Biotin)

Catalog No. ABIN2192140

Product Details anti-Nitrotyrosine Antibody (Biotin)

Target: Nitrotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nitrotyrosine antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA)

Sterility: 0.2 μm filtered

Clone: HM-11

Application Details

Application Notes: For immuno histology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Warning: keep sterile, do not add sodium azide or any nitric containing preservative since this will affect the antibody!

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-Nitrotyrosine antibody (Biotin) (ABIN2192140)

ter Steege, Koster-Kamphuis, van Straaten, Forget, Buurman: "Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA." in: Free radical biology & medicine, Vol. 25, Issue 8, pp. 953-63, (1999) (PubMed).

Kooy, Royall, Ye, Kelly, Beckman: "Evidence for in vivo peroxynitrite production in human acute lung injury." in: American journal of respiratory and critical care medicine, Vol. 151, Issue 4, pp. 1250-4, (1995) (PubMed).

Beckmann, Ye, Anderson, Chen, Accavitti, Tarpey, White: "Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry." in: Biological chemistry Hoppe-Seyler, Vol. 375, Issue 2, pp. 81-8, (1994) (PubMed).

Beckman, Beckman, Chen, Marshall, Freeman: "Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 4, pp. 1620-4, (1990) (PubMed).

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Abstract: Nitrotyrosine Products

Target Type: Chemical

Background: This highly specific monoclonal antibody HM.11 reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.