GLU I (PR-2) antibody

Catalog No. ABIN190719

Product Details

Target: GLU I (PR-2)

Reactivity: Nicotiana tabacum, Potato

Host: Rabbit

Clonality: Polyclonal

Application: Immunolocalization (IL), Western Blotting (WB)

Cross-Reactivity (Details): No cross-reactivity with: Arabidopsis thaliana

Characteristics: Expected / apparent Molecular Weight of the Antigene: 37 / 33 kDa

Purification: affinity purified

Immunogen: purified tobacco class I, basic ß-1,3-glucanase. Purified GLU I consists of a mixture of closely related polypeptides encoded by a family of GLU I genes comprising GLA B5APL3 derived from the sylvestris ancestor of tobacco, GLB P27666 derived from the tomentosiformis ancestor of tobacco and homeologous recombinants (Sperisen et al., 1991). Mature GLU I is processed from a pre-pro-polypeptide (Shinshi et al., 1988).

Isotype: IgG

Application Details

Application Notes: Recommended Dilution: 8 ug/mL with standard ECL (WB).

Comment:

Important note: for blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL of sterile water.

Buffer: PBS pH 7.4 (without Ca++)

Preservative: Without preservative

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References

Beffa, Neuhaus, Meins: "Physiological compensation in antisense transformants: specific induction of an \" glucan endo-1,3-beta-glucosidase in plants infected with necrotizing viruses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 19, pp. 8792-6, (1993) (PubMed).

Target Details

Target: GLU I (PR-2)

Background: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I and chitinase I contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis. Alternative names of the protein: basic beta-1,3-glucanase

Molecular Weight: expected: 37 kDa, apparent: 33 kDa

UniProt: P27666