CD19 antibody

Catalog No. ABIN457399

Product Details anti-CD19 Antibody

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD19 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: The rat monoclonal antibody 1D3 detects an extracellular epitope of murine CD19, 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells, it is lost on plasma cells.

Cross-Reactivity (Details): Mouse

Purification: Purified by protein-G affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Mouse CD19-transfected cell line

Clone: 1D3

Isotype: IgG2a

Application Details

Application Notes: Functional application: This antibody can induce down-regulation of CD19, affecting the proportions of B cell subpopulations.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD19 antibody (ABIN457399)

Shoham, Rajapaksa, Boucheix, Rubinstein, Poe, Tedder, Levy: "The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 8, pp. 4062-72, (2003) (PubMed).

Inabe, Kurosaki: "Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement." in: Blood, Vol. 99, Issue 2, pp. 584-9, (2002) (PubMed).

Cherukuri, Cheng, Pierce: "The role of the CD19/CD21 complex in B cell processing and presentation of complement-tagged antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 1, pp. 163-72, (2001) (PubMed).

Krop, de Fougerolles, Hardy, Allison, Schlissel, Fearon: "Self-renewal of B-1 lymphocytes is dependent on CD19." in: European journal of immunology, Vol. 26, Issue 1, pp. 238-42, (1996) (PubMed).

Krop, Shaffer, Fearon, Schlissel: "The signaling activity of murine CD19 is regulated during cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 1, pp. 48-56, (1996) (PubMed).

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19 (CD19 Products)

Synonyms: B4 antibody, CVID3 antibody, AW495831 antibody, CD19 antibody, CD19 molecule antibody, CD19 antigen antibody, CD19 antibody, Cd19 antibody

Background: CD19 Molecule,CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.,B4, Leu-12, Cvid

Gene ID: 12478

UniProt: P25918

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway