Gena antibody

Catalog No. ABIN3201020

Product Details

Target: Gena

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Characteristics: This product was purified from serum-free culture medium of the hybridoma clone TRA98 by proprietary chromatography processes under mild conditions.

Purification: Purified

Clone: TRA98

Isotype: IgG2a

Application Details

Application Notes: 1) Western blotting
2) Immunohistochemical staining

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 50 % Glycerol

Preservative: Azide free

Storage: -20 °C

References

Ohta, Yomogida, Dohmae, Nishimune: "Regulation of proliferation and differentiation in spermatogonial stem cells: the role of c-kit and its ligand SCF." in: Development (Cambridge, England), Vol. 127, Issue 10, pp. 2125-31, (2000) (PubMed).

Tanaka, Pereira, Nozaki, Tsuchida, Sawada, Mori, Nishimune: "A germ cell-specific nuclear antigen recognized by a monoclonal antibody raised against mouse testicular germ cells." in: International journal of andrology, Vol. 20, Issue 6, pp. 361-6, (1998) (PubMed).

Target Details

Target: Gena

Background: A monoclonal antibody TRA98 recognizes a mouse testicular germ cell-specific antigen. In adult tissues, bands of 60-100 kDa proteins are detected only in the testis by Western blotting analysis with TRA98. The signals are observed in male and female embryos after embryonic day 12.5. The signal in male is detected during development of germ cells and also after birth, but the signal in female disappears by 5 days after birth. The antigen is localized only in the nuclear fraction of testicular germ cells and this antibody can be used for immunohistochemical staining of testicular germ cells. The hybridoma producing TRA98 was obtained by immunization of a rat with the cell lysate of adult mouse testis by the group of Prof. Yoshitake Nishimune of Osaka University.