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Uncoating ATPase (AA 391-546) antibody

Reactivity: Cow, Hamster, Human, Mouse, Rat ICC, IHC, WB Host: Mouse Monoclonal 3C5 unconjugated
Catalog No. ABIN1742323
  • Target
    Uncoating ATPase
    Binding Specificity
    AA 391-546
    Reactivity
    Cow, Hamster, Human, Mouse, Rat
    Host
    Mouse
    Clonality
    Monoclonal
    Application
    Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    Specific for uncoating ATPase.
    Purification
    purified IgG
    Immunogen
    Purified bovine ATPase. Epitope Between aa 391-546.
    Clone
    3C5
    Isotype
    IgG1
  • Application Notes
    WB: 1 : 500 up to 1 : 5000 (AP staining)
    IP: not tested yet
    ICC: 1 : 500
    IHC-P: 1 : 200
    Comment

    For fixation a 20 min treatment with 3.7 % PFA in PBS is recommended. When microinjected into cells, the antibody inhibits clathrin mediated endocytosis.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
    Buffer
    PBS
    Handling Advice
    Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
    Storage
    -20 °C
    Storage Comment
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Wolf, Damme, Stroobants, DHooge, Beck, Hermans-Borgmeyer, Lüllmann-Rauch, Dierks, Lübke: "A mouse model for fucosidosis recapitulates storage pathology and neurological features of the milder form of the human disease." in: Disease models & mechanisms, Vol. 9, Issue 9, pp. 1015-28, (2016) (PubMed).

    Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

    Lee, Sharma, Südhof, Shen: "Synaptic function of nicastrin in hippocampal neurons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 24, pp. 8973-8, (2014) (PubMed).

    Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Walter, Kurps, de Wit, Schöning, Toft-Bertelsen, Lauks, Ziomkiewicz, Weiss, Schulz, Fischer von Mollard, Verhage, Sørensen: "The SNARE protein vti1a functions in dense-core vesicle biogenesis." in: The EMBO journal, Vol. 33, Issue 15, pp. 1681-97, (2014) (PubMed).

    García-Junco-Clemente, Cantero, Gómez-Sánchez, Linares-Clemente, Martínez-López, Luján, Fernández-Chacón: "Cysteine string protein-alpha prevents activity-dependent degeneration in GABAergic synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 21, pp. 7377-91, (2010) (PubMed).

    Burré, Sharma, Tsetsenis, Buchman, Etherton, Südhof: "Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro." in: Science (New York, N.Y.), Vol. 329, Issue 5999, pp. 1663-7, (2010) (PubMed).

    Hoopmann, Punge, Barysch, Westphal, Bückers, Opazo, Bethani, Lauterbach, Hell, Rizzoli: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).

    Sharma, Burré, Südhof: "CSPα promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity." in: Nature cell biology, Vol. 13, Issue 1, pp. 30-9, (2010) (PubMed).

    Newmyer, Schmid: "Dominant-interfering Hsc70 mutants disrupt multiple stages of the clathrin-coated vesicle cycle in vivo." in: The Journal of cell biology, Vol. 152, Issue 3, pp. 607-20, (2001) (PubMed).

    Hannan, Newmyer, Schmid: "ATP- and cytosol-dependent release of adaptor proteins from clathrin-coated vesicles: A dual role for Hsc70." in: Molecular biology of the cell, Vol. 9, Issue 8, pp. 2217-29, (1998) (PubMed).

    Höning, Kreimer, Robenek, Jockusch: "Receptor-mediated endocytosis is sensitive to antibodies against the uncoating ATPase (hsc70)." in: Journal of cell science, Vol. 107 ( Pt 5), pp. 1185-96, (1994) (PubMed).

  • Target
    Uncoating ATPase
    Background
    Synonyms: hsc 70
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