ICAM1 antibody

Catalog No. ABIN94177

Product Details anti-ICAM1 Antibody

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Human, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificity: The antibody MEM-111 reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation, it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.

Cross-Reactivity (Details): Rat, Human, Bovine

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Raji human Burkitt's lymphoma cell line

Clone: MEM-111

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Recommended dilution: 0.5-4 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: thymus, RE cells.
Western blotting: Positive control: RAJI cell line K562 leukemia cell line, JY cell line, aa ctivated T-lymphocytes, non-reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-ICAM1 antibody (ABIN94177)

Lee, Sieling, Ochoa, Krutzik, Guo, Hernandez, Rea, Cheng, Colonna, Modlin: "LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8128-36, (2007) (PubMed).

Burdick, McCaffery, Kim, Bochner, Konstantopoulos: "Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow." in: American journal of physiology. Cell physiology, Vol. 284, Issue 4, pp. C977-87, (2003) (PubMed).

Hertel, Lacaille, Strobl, Mellins, Mocarski: "Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus." in: Journal of virology, Vol. 77, Issue 13, pp. 7563-74, (2003) (PubMed).

Tachimoto, Kikuchi, Hudson, Bickel, Hamilton, Bochner: "Eotaxin-2 alters eosinophil integrin function via mitogen-activated protein kinases." in: American journal of respiratory cell and molecular biology, Vol. 26, Issue 6, pp. 645-9, (2002) (PubMed).

Bacáková, Mares, Lisá, Svorcík: "Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions." in: Biomaterials, Vol. 21, Issue 11, pp. 1173-9, (2000) (PubMed).

Nagaraju, Raben, Merritt, Loeffler, Kirk, Plotz: "A variety of cytokines and immunologically relevant surface molecules are expressed by normal human skeletal muscle cells under proinflammatory stimuli." in: Clinical and experimental immunology, Vol. 113, Issue 3, pp. 407-14, (1998) (PubMed).

Leeuwenberg, Smeets, Neefjes, Shaffer, Cinek, Jeunhomme, Ahern, Buurman: "E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro." in: Immunology, Vol. 77, Issue 4, pp. 543-9, (1993) (PubMed).

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: CD54 (ICAM1 Products)

Target Type: Viral Protein

Background: Intercellular adhesion molecule 1,CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.,ICAM-1, BB2, P3.58

Gene ID: 3383

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling