An antibody array can help you see what your ELISA has been missing..
Running an antibody array is like running dozens of ELISAs simultaneously. Arrays allow you to collect comprehensive, quantitative data on up to 40 different of related biomarkers at the same time, all while saving time, money, and precious sample volume.
Learn about how antibody arrays work, and read more about the benefits of antibody arrays below
How does an antibody array work?
Antibody arrays are fairly simple devices that operate on principles most researchers are already familiar with. The simplest way to explain an antibody array is to compare it to a common and familiar method, the ELISA.
In many ways, an antibody array is similar to an ELISA. Both arrays and ELISAs start with capture antibodies, affixed to a solid support. In an ELISA, the support structure is generally a 96-well polystyrene plate, but in an antibody array, it can be either a nitrocellulose membrane, or a glass microscope slide).
Like an ELISA, antibodies capture antigen out of an aqueous sample matrix (blood plasma, serum, and cell-culture media) are all common sample matrices analyzed via array and ELISA.
The main difference between an array and an ELISA is the diversity of antibodies on the support matrix, and by extension the number of biomarkers that can be analyzed simultaneously. ELISAs have the same capture antibody coated in each well and are capable of detecting only one biomarker at a time.
By contrast, an antibody array contains many different capture antibodies arranged in distinct spots. Antibody arrays are capable of capturing, distinguishing between, and detecting dozens of biomarkers simultaneously!
Do you need quantitative data on an exceptionally broad range of targets?
Multiplexed array sets like Human Cytokine Array Q600 combine multiple non-overlapping array slides, for analysis of up to 600 biomarkers at once.
During the detection phase of the assay, both arrays and ELISAs produce a quantifiable signal that corresponds to the amount of the respective biomarker in samples that have been assayed. ELISAs typically rely on a chromogenic substrate that changes color, and signal is detected and calculated by analysis on a spectrophotometric plate reader.
Antibody arrays may use either chemiluminescent signal (for nitrocellulose membrane based arrays), or fluorescent signal (for glass-slide based arrays). Data is collected using either a standard Gel-doc system, or a slide scanner respectively.
Note: antibodies-online and RayBiotech are proud to offer FREE slide scanning services for all glass-slide based RayBiotech arrays. After the wet-lab work is complete, simply box your array slide and send it in, and you'll receive your data back digitally!
Following detection and data collection, the signal generated is compared to a set of known-concentation standards and quantitative or semi-quantitative results are computed for on each biomarker analyzed.
Why is an array better than an ELISA?
While the antibody array can, in many ways, be compared to the ELISA, it is an undeniably superior platform for proteomic analysis. Unlike an ELISA, an array is able to provide quantitative data on up to several undred of different biomarkers simultaneously, but the benefits don't stop there!
Read more about how an array can help you improve your laboratory workflow below:
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