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|Application / Reactivity||Rat (Rattus)||Human|
|ELISA||9 Antibodies||32 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies||10 Antibodies|
|Immunofluorescence (IF)||5 Antibodies||18 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||4 Antibodies||12 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||8 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Western Blotting (WB)||20 Antibodies|
|Flow Cytometry (FACS)||6 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Antigen||Abl-Interactor 1 (ABI1) Antibodies|
|Epitope||AA 483-494, C-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This ABI1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-ABI1 AntibodyTarget Details ABI1 Application Details Handling Images
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Abl interactor 1(ABI1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: abl-interactor 1
Protein Name: Abl interactor 1
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SSH3BP1(483-494aa WYEGVCNRVTGL), different from the related rat and mouse sequences by one amino acid.|
Target Details ABI1Product Details anti-ABI1 Antibody Application Details Handling Images back to top
|Alternative Name||ABI1 (ABI1 Antibody Abstract)|
|Background||ABI1 is a human homolog of mouse Abl-interactor-1(Abi1), mapped on 10p11.2. ABI1 participates in the transduction of signals from Ras to Rac by regulating Rac-specific guanine nucleotide exchange factor(GEF) activities. ABI1 dramatically promoted ABL1-mediated tyrosine phosphorylation of MENA, but not of other substrates. Abi-1 regulates c-Abl-mediated phosphorylation of Mena by interacting with both proteins. ABI1 plays a role in the leukemogenesis by translocating to MLL.|
Application DetailsProduct Details anti-ABI1 Antibody Target Details ABI1 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ABI1 Antibody Target Details ABI1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-ABI1 Antibody Target Details ABI1 Application Details Handling back to top