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anti-Human Amphiregulin antibody for Immunohistochemistry (Frozen Sections)

Recommended Amphiregulin Antibody (supplied by: Log in to see )

Antigen
Amphiregulin (AREG) Antibodies
  • AR
  • AREG
  • AREG/SDGF
  • AREGB
  • CRDGF
  • SDGF
  • Sdgf
Epitope
AA 20-100
14
6
5
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
71
16
5
2
1
1
1
Host
Rabbit
62
10
2
2
1
1
Clonality
Polyclonal
Conjugate
This Amphiregulin antibody is un-conjugated
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
47
24
22
15
14
5
4
4
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1858070
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088003743 ABIN3201206 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 20-100 Log in to see

Similar anti-Amphiregulin Antibodies

Application / Reactivity Human
ELISA 22 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 22 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies
Immunoprecipitation (IP) 15 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 43 Antibodies

General

Antigen Amphiregulin (AREG) Antibodies
Epitope AA 20-100
(14), (6), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(71), (16), (5), (2), (1), (1), (1)
Host Rabbit
(62), (10), (2), (2), (1), (1)
Clonality
Polyclonal
Conjugate This Amphiregulin antibody is un-conjugated
(1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(47), (24), (22), (15), (14), (5), (4), (4), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Amphiregulin Antibody

Target Details Amphiregulin Application Details Handling Images
Sequence MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -S GHYAAGLDLN DTYSGKREPF SGDHSADGFE VTSRSEMSSG SEISPVSEMP SSSEPSSGAD YDYSEEYDNE PQIPGYIVDD
Specificity It has been selected for its ability to recognize AREG in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen AREG (AA 20-100)
Isotype IgG

Target Details Amphiregulin

Product Details anti-Amphiregulin Antibody Application Details Handling Images back to top
Antigen
Pathways RTK Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-Amphiregulin Antibody Target Details Amphiregulin Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant AREG (Ser20~Asp100) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-Amphiregulin Antibody Target Details Amphiregulin Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Amphiregulin Antibody Target Details Amphiregulin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Amphiregulin antibody (AREG) (AA 20-100) (ABIN1858070) anti-Amphiregulin (AREG) (AA 20-100) antibody