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anti-Mouse (Murine) Angiopoietin 1 antibody for Enzyme Immunoassay

Recommended Angiopoietin 1 Antibody (supplied by: Log in to see )

Antigen
Angiopoietin 1 (ANGPT1) Antibodies
  • 1110046O21Rik
  • AGP1
  • AGPT
  • Agpt
  • Agpt1
  • Ang-1
  • ANG-1
  • ANG1
  • Ang1
  • ang1
  • angpt1
  • DHCR14A
  • MGC80788
  • NET47
  • wu:fc76g06
Epitope
N-Term, AA 21-40
26
16
13
10
9
8
7
6
5
5
5
5
5
4
4
3
3
3
2
2
2
1
1
Reactivity
Human, Mouse (Murine)
129
60
36
18
16
5
2
1
1
Host
Rabbit
146
15
10
1
Clonality
Polyclonal
Conjugate
This Angiopoietin 1 antibody is un-conjugated
17
14
7
3
3
3
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
128
81
32
29
11
11
9
5
2
2
2
Supplier
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Catalog No. ABIN118022
$ 500.50
Plus shipping costs $45.00

Top referenced anti-Angiopoietin 1 antibody for Enzyme Immunoassay

Similar anti-Angiopoietin 1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 26 Antibodies 56 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 3 Antibodies
Immunochromatography (IC) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 6 Antibodies
Immunofluorescence (IF) 3 Antibodies 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 13 Antibodies 27 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Western Blotting (WB) 43 Antibodies

General

Antigen Angiopoietin 1 (ANGPT1) Antibodies
Epitope N-Term, AA 21-40
(26), (16), (13), (10), (9), (8), (7), (6), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1)
Reactivity Human, Mouse (Murine)
(129), (60), (36), (18), (16), (5), (2), (1), (1)
Host Rabbit
(146), (15), (10), (1)
Clonality
Polyclonal
Conjugate This Angiopoietin 1 antibody is un-conjugated
(17), (14), (7), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(128), (81), (32), (29), (11), (11), (9), (5), (2), (2), (2)
Pubmed 2 references available
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Product Details anti-Angiopoietin 1 Antibody

Target Details Angiopoietin 1 Application Details Handling References for anti-Angiopoietin 1 Antibody (ABIN118022) Images
Sequence N-Q-R-R-N-P-E-N-G-G-R-R-Y-N-R-I-Q-H-G-Q
Purification Serum
Immunogen This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a region (aa 21-40) near the N-terminus of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Target Details Angiopoietin 1

Product Details anti-Angiopoietin 1 Antibody Application Details Handling References for anti-Angiopoietin 1 Antibody (ABIN118022) Images back to top
Antigen
Alternative Name Angiopoietin-1 (ANGPT1 Antibody Abstract)
Background Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, Ang-1 is also expressed in the heart and liver. Anti-Ang 1 and anti-Ang 2 antibodies recognize a band above 55 kDa. Both antibodies show banding at 75 kDa which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher that the calculated molecular weight of 55 kD.Synonyms: ANG-1, ANG1, ANGPT1, KIAA0003
Gene ID 10090
UniProt O08538
Research Area Growth Factors
Pathways RTK Signaling

Application Details

Product Details anti-Angiopoietin 1 Antibody Target Details Angiopoietin 1 Handling References for anti-Angiopoietin 1 Antibody (ABIN118022) Images back to top
Application Notes Suitable for Western blotting (1/500) and other antibody based assays. The reaction of this antiserum directly with cell supernatants may result in highbackground due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody: antigen complex and thendetecting the antigen. This method results in very clean and strong signal. This product was assayed by Immunoblot against supernatants of angiopoietinexpressingendothelial cells from human and mouse sources. A single band of the expected apparentmolecular weight was observed at a 1/500 dilution. Recommended Dilutions: ELISA: 1/5,000-1/25,000. Western Blot: 1/500-1/2,000.
Restrictions For Research Use only

Handling

Product Details anti-Angiopoietin 1 Antibody Target Details Angiopoietin 1 Application Details References for anti-Angiopoietin 1 Antibody (ABIN118022) Images back to top
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice . Avoid cycles of freezing and thawing.
Storage -20 °C
Storage Comment Store vial at -20° C or below prior to opening. For extended storage aliquot contents and freeze at -20 °C or below.

References for anti-Angiopoietin 1 Antibody (ABIN118022)

Product Details anti-Angiopoietin 1 Antibody Target Details Angiopoietin 1 Application Details Handling Images back to top
Background publications

Suri, Jones, Patan, Bartunkova, Maisonpierre, Davis, Sato, Yancopoulos: "Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis." in: Cell, Vol. 87, Issue 7, pp. 1171-80, 1997

Davis, Aldrich, Jones, Acheson, Compton, Jain, Ryan, Bruno, Radziejewski, Maisonpierre, Yancopoulos: "Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning." in: Cell, Vol. 87, Issue 7, pp. 1161-9, 1997

Images

Product Details anti-Angiopoietin 1 Antibody Target Details Angiopoietin 1 Application Details Handling References for anti-Angiopoietin 1 Antibody (ABIN118022) back to top
Supplier Images
Western Blotting (WB) image for anti-Angiopoietin 1 antibody (ANGPT1) (N-Term) (ABIN118022) Immunoblotting. Rabbit anti-Ang 1 was used at a 1:500 dilution to detect mouse Ang 1 ...