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anti-Mouse (Murine) Angiopoietin 1 antibody for Western Blotting

Recommended Angiopoietin 1 Antibody (supplied by: Log in to see )

Antigen
Angiopoietin 1 (ANGPT1) Antibodies
  • ang1
  • wu:fc76g06
  • ANG-1
  • angpt1
  • MGC80788
  • 1110046O21Rik
  • Ang-1
  • Ang1
  • AGP1
  • AGPT
  • ANG1
  • Agpt
  • Agpt1
  • DHCR14A
  • NET47
  • angiopoietin 1
  • angiopoietin-1
  • angiopoietin-like 1
  • transmembrane 7 superfamily member 2
  • angpt1
  • ANG-1
  • Angpt1
  • ANGPT1
  • ANGPTL1
  • TM7SF2
Epitope
N-Term, AA 21-40
20
19
15
12
8
7
7
6
5
5
5
5
5
5
4
4
4
3
3
3
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
139
72
46
24
19
9
6
4
3
2
1
1
1
1
1
1
1
Host
Rabbit
151
16
11
1
Clonality
Polyclonal
Conjugate
This Angiopoietin 1 antibody is un-conjugated
17
14
7
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
153
88
46
30
13
9
9
8
3
3
2
2
Supplier
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Catalog No. ABIN118022
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1870847 ABIN95109 IHC WB Rabbit Log in to see Polyclonal 2
3.1870847 ABIN1043685 IHC WB Rabbit N-Term Log in to see Polyclonal 1
3.1870847 ABIN728323 IF (p) IHC (p) WB Rabbit IgG AA 280-330 Log in to see Polyclonal 4
3.1870847 ABIN2789373 WB Rabbit C-Term Log in to see Polyclonal
3.1870847 ABIN2789374 WB Rabbit N-Term Log in to see Polyclonal
3.1870847 ABIN4902908 IF WB Rabbit IgG Log in to see Polyclonal
3.1870847 ABIN1857990 ICC IHC IP WB Rabbit IgG AA 35-231 Log in to see Polyclonal
3.1870847 ABIN1171922 ICC IHC IP WB Rabbit IgG AA 253-429 Log in to see Polyclonal
3.1870847 ABIN2561113 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
3.1870847 ABIN285661 ELISA WB Rabbit N-Term Log in to see Polyclonal
3.1870847 ABIN3029948 IHC (p) ELISA WB Rabbit IgG AA 225-239 Log in to see Polyclonal
1 ABIN962636 IHC (p) ELISA WB Goat AA 76-85 Log in to see Polyclonal
1 ABIN799271 WB Rabbit N-Term Log in to see Polyclonal
1 ABIN218573 IHC (p) ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2470306 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN790997 IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2736277 WB Rabbit IgG Log in to see Polyclonal
1 ABIN326794 WB Rabbit IgG AA 21-40, N-Term Log in to see Polyclonal
1 ABIN2755625 IC IF WB Rabbit Center Log in to see Polyclonal
1 ABIN3183282 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal

Top referenced anti-Angiopoietin 1 antibody for Western Blotting

Similar anti-Angiopoietin 1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 30 Antibodies 58 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 3 Antibodies
Immunochromatography (IC) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 4 Antibodies
Immunofluorescence (IF) 3 Antibodies 3 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 57 Antibodies

General

Antigen Angiopoietin 1 (ANGPT1) Antibodies
Epitope N-Term, AA 21-40
(20), (19), (15), (12), (8), (7), (7), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(139), (72), (46), (24), (19), (9), (6), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(151), (16), (11), (1)
Clonality
Polyclonal
Conjugate This Angiopoietin 1 antibody is un-conjugated
(17), (14), (7), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(153), (88), (46), (30), (13), (9), (9), (8), (3), (3), (2), (2)
Pubmed 2 references available
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Product Details anti-Angiopoietin 1 Antibody

Target Details Angiopoietin 1 Application Details Handling References for anti-Angiopoietin 1 Antibody (ABIN118022) Images
Sequence N-Q-R-R-N-P-E-N-G-G-R-R-Y-N-R-I-Q-H-G-Q
Purification Serum
Immunogen This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a region (aa 21-40) near the N-terminus of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Target Details Angiopoietin 1

Product Details anti-Angiopoietin 1 Antibody Application Details Handling References for anti-Angiopoietin 1 Antibody (ABIN118022) Images back to top
Antigen
Alternative Name Angiopoietin-1 (ANGPT1 Antibody Abstract)
Background Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, Ang-1 is also expressed in the heart and liver. Anti-Ang 1 and anti-Ang 2 antibodies recognize a band above 55 kDa. Both antibodies show banding at 75 kDa which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher that the calculated molecular weight of 55 kD.Synonyms: ANG-1, ANG1, ANGPT1, KIAA0003
Gene ID 10090
UniProt O08538
Research Area Growth Factors
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process

Application Details

Product Details anti-Angiopoietin 1 Antibody Target Details Angiopoietin 1 Handling References for anti-Angiopoietin 1 Antibody (ABIN118022) Images back to top
Application Notes Suitable for Western blotting (1/500) and other antibody based assays. The reaction of this antiserum directly with cell supernatants may result in highbackground due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody: antigen complex and thendetecting the antigen. This method results in very clean and strong signal. This product was assayed by Immunoblot against supernatants of angiopoietinexpressingendothelial cells from human and mouse sources. A single band of the expected apparentmolecular weight was observed at a 1/500 dilution. Recommended Dilutions: ELISA: 1/5,000-1/25,000. Western Blot: 1/500-1/2,000.
Restrictions For Research Use only

Handling

Product Details anti-Angiopoietin 1 Antibody Target Details Angiopoietin 1 Application Details References for anti-Angiopoietin 1 Antibody (ABIN118022) Images back to top
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice . Avoid cycles of freezing and thawing.
Storage -20 °C
Storage Comment Store vial at -20° C or below prior to opening. For extended storage aliquot contents and freeze at -20 °C or below.

References for anti-Angiopoietin 1 Antibody (ABIN118022)

Product Details anti-Angiopoietin 1 Antibody Target Details Angiopoietin 1 Application Details Handling Images back to top
Background publications

Suri, Jones, Patan, Bartunkova, Maisonpierre, Davis, Sato, Yancopoulos: "Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis." in: Cell, Vol. 87, Issue 7, pp. 1171-80, 1997

Davis, Aldrich, Jones, Acheson, Compton, Jain, Ryan, Bruno, Radziejewski, Maisonpierre, Yancopoulos: "Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning." in: Cell, Vol. 87, Issue 7, pp. 1161-9, 1997

Images

Product Details anti-Angiopoietin 1 Antibody Target Details Angiopoietin 1 Application Details Handling References for anti-Angiopoietin 1 Antibody (ABIN118022) back to top
Supplier Images
Western Blotting (WB) image for anti-Angiopoietin 1 antibody (ANGPT1) (N-Term) (ABIN118022) Immunoblotting. Rabbit anti-Ang 1 was used at a 1:500 dilution to detect mouse Ang 1 ...