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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||7 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||5 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies|
|Immunohistochemistry (IHC)||29 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||14 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Western Blotting (WB)||60 Antibodies|
|Antigen||Angiopoietin 2 (ANGPT2) Antibodies|
|Epitope||AA 180-283 Alternatives|
|Conjugate||This Angiopoietin 2 antibody is un-conjugated Alternatives|
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Angiopoietin 2 AntibodyTarget Details Angiopoietin 2 Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Angiopoietin-2(ANGPT2) detection. Tested with WB, IHC-P, ELISA in Human.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Angiopoietin-2(ANGPT2) detection. Tested with WB, IHC-P, ELISA in Human.
Gene Name: angiopoietin 2
Protein Name: Angiopoietin-2
|Purification||Immunogen affinity purified.|
|Immunogen||E. coli-derived human Angiopoietin-2 recombinant protein (Position: E180-D283). Human Angiopoietin-2 shares 75% and 77.9% amino acid (aa) sequence identity with mouse and rat Angiopoietin-2, respectively.|
Target Details Angiopoietin 2Product Details anti-Angiopoietin 2 Antibody Application Details Handling Images back to top
|Alternative Name||ANGPT2 (ANGPT2 Antibody Abstract)|
ANGPT2, also known as ANG2 or Angiopoietin 2, is a protein that in humans is encoded by the ANGPT2 gene. It is mapped to 8p23.1. ANGPT2 is a naturally occurring antagonist of ANG1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. ANGPT2 was significantly increased in plasma and alveolar edema fluid in adults with acute lung injury compared to controls or patients with hydrostatic pulmonary edema, tracheal. ANGPT2 was also significantly increased in neonates with respiratory distress syndrome who developed bronchopulmonary edema. It is also a mediator of epithelial necrosis with an important role in hyperoxic acute lung injury and pulmonary edema.
Synonyms: ANG 2 | ANG2 | ANG-2 | Angiopoietin 2 | Angiopoietin2 | Angiopoietin-2 | Angiopoietin2(ANG2) | Angiopoietin-2(ANG-2) | ANGP2 | ANGPT2 | O15123
Application DetailsProduct Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.