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anti-Human Angiopoietin 2 antibody for Immunohistochemistry (Frozen Sections)

Recommended Angiopoietin 2 Antibody (supplied by: Log in to see )

Antigen
Angiopoietin 2 (ANGPT2) Antibodies
  • AGPT2
  • Agpt2
  • ANG-2
  • Ang-2
  • ANG2
  • ang2
  • Ang2
  • ANGIOPOIETIN-2
  • ANGPT2
  • angpt2
Reactivity
Human
85
58
32
17
2
2
2
2
1
1
1
Host
Mouse
107
8
2
2
1
1
Clonality
Monoclonal
Conjugate
This Angiopoietin 2 antibody is un-conjugated
11
8
8
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
84
54
32
21
11
11
9
8
3
3
2
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Catalog No. ABIN259881
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008841162 ABIN1857996 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 269-496 Log in to see Polyclonal
0.0008841162 ABIN1171925 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 24-165 Log in to see Polyclonal

Similar anti-Angiopoietin 2 Antibodies

Application / Reactivity Human
ELISA 32 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 26 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 60 Antibodies

General

Antigen Angiopoietin 2 (ANGPT2) Antibodies
Reactivity Human
(85), (58), (32), (17), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(107), (8), (2), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Angiopoietin 2 antibody is un-conjugated
(11), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(84), (54), (32), (21), (11), (11), (9), (8), (3), (3), (2)
Pubmed 2 references available
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Product Details anti-Angiopoietin 2 Antibody

Target Details Angiopoietin 2 Application Details Handling References for anti-Angiopoietin 2 Antibody (ABIN259881) Images
Specificity This recognizes human Ang2 without cross reactivity with human Ang1.
Purification Protein G purified
Immunogen purified human recombinant Ang2
Clone MM0020-1F29
Isotype IgG2

Target Details Angiopoietin 2

Product Details anti-Angiopoietin 2 Antibody Application Details Handling References for anti-Angiopoietin 2 Antibody (ABIN259881) Images back to top
Antigen
Alternative Name Angiopoietin-2 (ANGPT2 Antibody Abstract)
Background Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, byregulating tyrosine phosphorylation of the membrane receptor Tie 2/Tek. Angiopoietin 2is only 60% homologous with Angiopoietin 1. Angiopoietin 1 binding to Tie 2/Tek causesphosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thusblocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascularremodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus. Alternate Names: Anti-AGPT 2 antibody, Anti-Agpt2 antibody, Anti-ANG 2 antibody, Anti-ANG2 antibody,Anti-Angiopoietin 2a antibody, Anti-Angiopoietin 2B antibody, Anti-Angiopoietin2antibody, Anti-ANGPT 2 antibody, Anti-ANGPT2 antibody, Anti-Tie2 ligand antibody.
Gene Symbol: ANGPT2
Gene ID 285
Research Area Growth Factors
Pathways RTK Signaling

Application Details

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Handling References for anti-Angiopoietin 2 Antibody (ABIN259881) Images back to top
Application Notes By Western blot, this antibody detects a band at ~60-75 kDa under reducing conditions. Has been reported to work in Immunohistochemistry-Frozen (PMID 22302773) and Immunofluorescence in a customer review.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:25, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Frozen 1:10-1:2000, Immunohistochemistry-Paraffin 1:100-200, Western Blot 1:500-1:1000
Restrictions For Research Use only

Handling

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Application Details References for anti-Angiopoietin 2 Antibody (ABIN259881) Images back to top
Format Lyophilized
Reconstitution Reconstitute the antibody with 500 µL sterile 1 x PBS (or with 40 µL H2O plus 460 µL PBS), to give a final concentration of 200 µg/mL. Alternatively, reconstitute the antibody in 40 µL H2O if a small volume is preferred.
Buffer Lyophilized from a 0.2 µm filtered solution in Phosphate-buffered saline (PBS).
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.

References for anti-Angiopoietin 2 Antibody (ABIN259881)

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Application Details Handling Images back to top
Product cited in:

de Vries, Khairoun, Lindeman, Bajema, de Heer, Roest, van Zonneveld, van Kooten, Rabelink, Schaapherder, Reinders: "Renal ischemia-reperfusion induces release of angiopoietin-2 from human grafts of living and deceased donors." in: Transplantation, Vol. 96, Issue 3, pp. 282-9, 2013 (Sample species: Human). Further details: Immunohistochemistry (Frozen Sections)

Laquer, Dao, Pavlis, Nguyen, Chen, Harris, Rugg, Kelly: "Immunohistochemistry of angiogenesis mediators before and after pulsed dye laser treatment of angiomas." in: Lasers in surgery and medicine, Vol. 44, Issue 3, pp. 205-10, 2012

Images

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Application Details Handling References for anti-Angiopoietin 2 Antibody (ABIN259881) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Angiopoietin 2 antibody (ANGPT2) (ABIN259881) Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody (MM0020-1F29) [ABIN259881] - N...