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anti-Mouse (Murine) Angiopoietin 2 antibody for Western Blotting

Recommended Angiopoietin 2 Antibody (supplied by: Log in to see )

Antigen
Angiopoietin 2 (ANGPT2) Antibodies
  • AGPT2
  • Agpt2
  • ANG-2
  • Ang-2
  • ANG2
  • ang2
  • Ang2
  • ANGIOPOIETIN-2
  • ANGPT2
  • angpt2
Epitope
N-Term, AA 21-40
20
13
9
7
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
81
56
32
17
2
2
2
2
1
1
1
Host
Rabbit
104
7
2
2
1
1
Clonality
Polyclonal
Conjugate
This Angiopoietin 2 antibody is un-conjugated
11
8
8
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
80
54
29
19
11
10
9
8
3
2
2
Supplier
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Catalog No. ABIN118023
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN654062 FACS IHC (p) WB Rabbit Ig AA 404-432, C-Term Log in to see Polyclonal 3
0.12303223 ABIN1450125 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 5
0.12303223 ABIN95113 WB Rabbit N-Term Log in to see Polyclonal 1
0.12303223 ABIN1043684 WB Rabbit N-Term Log in to see Polyclonal 1
0.12303223 ABIN1105366 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.12303223 ABIN2705461 WB Rabbit Center Log in to see Polyclonal
0.12303223 ABIN2789397 WB Rabbit Middle Region Log in to see Polyclonal
0.12303223 ABIN2854896 IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.12303223 ABIN1870971 IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN3021112 IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN1171928 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 19-147 Log in to see Polyclonal
0.12303223 ABIN1030235 ELISA WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN2421171 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2403674 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011806369 ABIN671781 IF (p) IHC (p) WB Rabbit IgG AA 450-490 Log in to see Polyclonal 2
0.0011806369 ABIN1981408 FACS IHC WB Rabbit IgG AA 404-432, C-Term Log in to see Polyclonal
0.0011806369 ABIN289374 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011806369 ABIN671790 IHC (p) WB HRP Rabbit IgG AA 450-490 Log in to see Polyclonal
0.0011806369 ABIN671783 IHC (p) WB Biotin Rabbit IgG AA 450-490 Log in to see Polyclonal
0.0011806369 ABIN1991365 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal

Top referenced anti-Angiopoietin 2 antibody for Western Blotting

Similar anti-Angiopoietin 2 Antibodies

Application / Reactivity Mouse (Murine) Human
Western Blotting (WB) 38 Antibodies 57 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 23 Antibodies 14 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunofluorescence (IF) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 4 Antibodies
Flow Cytometry (FACS) 8 Antibodies 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies

General

Antigen Angiopoietin 2 (ANGPT2) Antibodies
Epitope N-Term, AA 21-40
(20), (13), (9), (7), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(81), (56), (32), (17), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(104), (7), (2), (2), (1), (1)
Clonality
Polyclonal
Conjugate This Angiopoietin 2 antibody is un-conjugated
(11), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(80), (54), (29), (19), (11), (10), (9), (8), (3), (2), (2)
Pubmed 2 references available
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Product Details anti-Angiopoietin 2 Antibody

Target Details Angiopoietin 2 Application Details Handling References for anti-Angiopoietin 2 Antibody (ABIN118023) Images
Sequence N-N-F-R-K-S-V-D-S-T-G-R-R-Q-Y-Q-V-Q-N-G-P Remarks: A residue of cysteine was added to the amino terminal end to facilitate coupling.
Specificity This antiserum is directed against mouse angiopoietin 2 and shows no reactivity with angiopoietin 1 on mouse derived proteins. A 75 kDa band is detected when assayed by Immunoblot. This reagent cross reacts with human angiopoietin 2. Partially cross reactivity is noted with human angiopoietin 1.
Purification Serum
Immunogen This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region (aa 21-40) near the N-terminus of mouse angiopoietin 2 protein conjugated to KLH using maleimide.

Target Details Angiopoietin 2

Product Details anti-Angiopoietin 2 Antibody Application Details Handling References for anti-Angiopoietin 2 Antibody (ABIN118023) Images back to top
Antigen
Alternative Name Angiopoietin-2 (ANGPT2 Antibody Abstract)
Background Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus. Anti-Ang 1 and anti-Ang 2 antibodies recognize a band above 55 kDa. Both antibodies show banding at 75 kDa which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher that the calculated molecular weight of 55 kD.Synonyms: ANG-2, ANG2, ANGPT2
Gene ID 11601
NCBI Accession NP_031452
UniProt O35608
Research Area Growth Factors
Pathways RTK Signaling

Application Details

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Handling References for anti-Angiopoietin 2 Antibody (ABIN118023) Images back to top
Application Notes Suitable for Western blotting (1: 500) and other antibody based assays. The reaction of this antiserum directly with cell supernatants may result in highbackground due to reactivity of components in the serum. This can be alleviated by firstimmunoprecipitating the antibody: antigen complex and then detecting the antigen. Thismethod results in very clean and strong signal. Recommended Dilutions: This product was assayed by immunoblot against supernatantsof angiopoietin expressing endothelial cells from human and mouse sources. A singleband of the expected apparent molecular weight was observed at a 1: 500 dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Application Details References for anti-Angiopoietin 2 Antibody (ABIN118023) Images back to top
Format Liquid
Concentration 85.0 mg/mL (by refractometry)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.09 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Angiopoietin 2 Antibody (ABIN118023)

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Application Details Handling Images back to top
Background publications

Maisonpierre, Suri, Jones, Bartunkova, Wiegand, Radziejewski, Compton, McClain, Aldrich, Papadopoulos, Daly, Davis, Sato, Yancopoulos: "Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis." in: Science (New York, N.Y.), Vol. 277, Issue 5322, pp. 55-60, 1997

Suri, Jones, Patan, Bartunkova, Maisonpierre, Davis, Sato, Yancopoulos: "Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis." in: Cell, Vol. 87, Issue 7, pp. 1171-80, 1997

Images

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Application Details Handling References for anti-Angiopoietin 2 Antibody (ABIN118023) back to top
Supplier Images
Western Blotting (WB) image for anti-Angiopoietin 2 antibody (ANGPT2) (N-Term) (ABIN118023) Immunoblotting. Rabbit anti-Ang 2 was used at a.,.1:500 dilution to detect mouse Ang ...