You are viewing an incomplete version of our website. Please click to reload the website as full version.

Human Angiopoietin 2 ELISA Kit

Recommended Angiopoietin 2 Product (supplied by: Log in to see )

Antigen
Angiopoietin 2 (ANGPT2) ELISA Kits
  • ang2
  • angpt2
  • ANG-2
  • ANGPT2
  • Agpt2
  • Ang-2
  • Ang2
  • AGPT2
  • ANG2
  • ANGIOPOIETIN-2
  • angiopoietin 2b
  • angiopoietin-2
  • angiopoietin 2
  • angpt2b
  • ANG-2
  • ANGPT2
  • LOC100225992
  • Angpt2
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
23
13
9
9
7
4
3
2
2
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
10-3000 pg/mL
Minimum Detection Limit
10 pg/mL
Application
ELISA
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN624940
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
12.876543 ABIN414354 Sandwich ELISA Serum, Plasma, Biological Fluids 12.5-800 pg/mL 12.5 pg/mL Log in to see 3
12.876543 ABIN1672763 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Milk 156-10000 pg/mL 156 pg/mL Log in to see 3
12.876543 ABIN365001 Sandwich ELISA Serum, Plasma, Tissue Homogenate 1.25-80 ng/mL 1.25 ng/mL Log in to see
12.876543 ABIN924873 Sandwich ELISA Cell Lysate, Serum, Plasma 94-6000 pg/mL 94 pg/mL Log in to see
12.876543 ABIN1113387 Sandwich ELISA Serum, Plasma, Biological Fluids 46.875-3000 pg/mL 46.875 pg/mL Log in to see
12.876543 ABIN3198394 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 15.63-1000 pg/mL 15.63 pg/mL Log in to see
12.876543 ABIN454257 Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
12.876543 ABIN1888483 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine 10-3000 pg/mL 10 pg/mL Log in to see
12.876543 ABIN2708072 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 156-10000 pg/mL 156 pg/mL Log in to see
1 ABIN3032889 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4.1-3000 pg/mL 4.1 pg/mL Log in to see
1 ABIN1568361 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.781-50 ng/mL 0.781 ng/mL Log in to see
1 ABIN1112556 Sandwich ELISA Serum, Plasma, Urine, Tissue Samples, Cell Culture Supernatant 8-150 pg/mL 8 pg/mL Log in to see
1 ABIN2042346 Sandwich ELISA 6-10000 pg/mL 6 pg/mL Log in to see
1 ABIN2065294 Cell Lysate, Serum, Plasma 94-6000 pg/mL 94 pg/mL Log in to see
1 ABIN2683443 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Milk, Saliva, Serum 156-10000 pg/mL 156 pg/mL Log in to see
1 ABIN2683444 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Samples, Urine 8-150 pg/mL 8 pg/mL Log in to see
1 ABIN2541289 Serum, Plasma, Saliva, Biological Fluids 3-150 ng/L 3 ng/L Log in to see
1 ABIN2115876 7.8-500 pg/mL 7.8 pg/mL Log in to see
1 ABIN2683445 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine Log in to see
1 ABIN2541293 Sandwich ELISA Serum, Plasma, Saliva, Biological Fluids 12.5-800 pg/mL 12.5 pg/mL Log in to see

Top referenced Angiopoietin 2 ELISA Kit for ELISA

Similar Angiopoietin 2 ELISA Kits

Application / Reactivity Human
ELISA 22 ELISA Kits

General

Antigen Angiopoietin 2 (ANGPT2) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(23), (13), (9), (9), (7), (4), (3), (2), (2), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 10-3000 pg/mL
Minimum Detection Limit 10 pg/mL
Application ELISA
Pubmed 8 references available
Supplier Log in to see

Product Details Angiopoietin 2 ELISA Kit

Target details Application Details Handling References for Angiopoietin 2 Kit (ABIN624940) Images
Purpose Human Angiopoietin-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, Angiopoietin-1, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, GCSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivity < 10 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details Angiopoietin 2 ELISA Kit Application Details Handling References for Angiopoietin 2 Kit (ABIN624940) Images back to top
Antigen
Alternative Name Angiopoietin-2 (ANGPT2 ELISA Kit Abstract)
Background The Human Angiopoietin-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Angiopoietin-2 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human Angiopoietin-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Angiopoietin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
Gene ID 285
UniProt O15123
Research Area Growth Factors
Pathways RTK Signaling

Application Details

Product Details Angiopoietin 2 ELISA Kit Target details Handling References for Angiopoietin 2 Kit (ABIN624940) Images back to top
Application Notes Recommended Dilution for serum and plasma samples3 - 20 fold
Sample Volume 100 μL
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: If your samples need to be diluted, 1x Assay Diluent (Item E) should be used for dilution of serum/plasma/culture supernatants/urine. Suggested dilution for normal serum/plasma: 3-20 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent (Item E) should be diluted 5-fold with deionized or distilled water before use.
4. Preparation of standard: Briefly spin the vial of Item C. Add 400 µL 1x Assay Diluent (Item E) into Item C vial to prepare a 130 ng/mL standard solution. Dissolve the powder thoroughly by a gentle mix. Add 15 µL Angiopoietin-2 standard from the vial of tem C, into a tube with 635 µL 1x Assay Diluent to prepare a 3000 pg/mL standard solution. Pipette 400myl 1x Assay Diluent into each tube. Use the stock standard solution to produce a Dilution series . Mix each tube thoroughly before the next transfer. 1x Assay Diluent serves as the zero standard (0 pg/mL). 200 µL 15 µL standard + 635 µL 200myl 200 µL 200 µL 200 µL 200 µL 3000 1000 333.3 111.1 37.04 12.35 4.12 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 200-fold with 1x Assay Diluent. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 50 µL of HRP-Streptavidin concentrate into a tube with 10 ml 1x Assay Diluent to prepare a 200-fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent Angiopoietin-2 concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.001 0.01 0.1 1 10
Sensitivity: The minimum detectable dose of Angiopoietin-2 is typically less than 10 pg/mL.
Recovery: Recovery was determined by spiking various levels of Angiopoietin-2 into normal human serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 85.3 82-109 Plasma 82.1 95-116 Cell culture media 96.9 76-92
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 110.3 109.4 109.2 Range ( %) 99-118 100-117 98-119 1:4 Average % of Expected 113.5 112.2 111.6 Range ( %) 103-120 102-120 101-121
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only

Handling

Product Details Angiopoietin 2 ELISA Kit Target details Application Details References for Angiopoietin 2 Kit (ABIN624940) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

References for Angiopoietin 2 Kit (ABIN624940)

Product Details Angiopoietin 2 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Chen, Li, Zhang, Li, Wang, Guo, Luo, Wang: "Urinary angiopoietin-2 is associated with albuminuria in patients with type 2 diabetes mellitus." in: International journal of endocrinology, Vol. 2015, pp. 163120, 2015

Köksal, Ozbek, Bayraktar, Yazici: "Evaluation of angiopoietin 1 and 2, vascular endothelial growth factor, and tumor necrosis factor alpha levels in asthmatic children." in: Allergy and asthma proceedings : the official journal of regional and state allergy societies, Vol. 35, Issue 6, pp. 482-8, 2015

Palud, Parmentier-Decrucq, Pastre, De Freitas Caires, Lassalle, Mathieu: "Evaluation of endothelial biomarkers as predictors of organ failures in septic shock patients." in: Cytokine, Vol. 73, Issue 2, pp. 213-8, 2015

Pul, Yildiz, Morbiducci, Skripuletz, Schwenkenbecher, Stangel, Götz, Berding, Trebst, Donnerstag: "CSF Levels of Angiopoietin-2 Do Not Differ between Patients with CSF Fluid Leakage Syndrome and Controls." in: Disease markers, Vol. 2015, pp. 343818, 2015

Koob, Lim, Massee, Zabek, Rennert, Gurtner, Li: "Angiogenic properties of dehydrated human amnion/chorion allografts: therapeutic potential for soft tissue repair and regeneration." in: Vascular cell, Vol. 6, pp. 10, 2014

Vizio, Biasi, Scirelli, Novarino, Prati, Ciuffreda, Montrucchio, Poli, Bellone: "Pancreatic-carcinoma-cell-derived pro-angiogenic factors can induce endothelial-cell differentiation of a subset of circulating CD34+ progenitors." in: Journal of translational medicine, Vol. 11, pp. 314, 2013

Bhaskar, Gupta, Sreenivas, Rani, Kumar, Sharma, Sharma, Sharma, Thakur: "Synergistic effect of vascular endothelial growth factor and angiopoietin-2 on progression free survival in multiple myeloma." in: Leukemia research, Vol. 37, Issue 4, pp. 410-5, 2013

Freeman: "A perspective metric for visual slant and shape." in: Psychologische Forschung, Vol. 32, Issue 4, pp. 296-323, 1970

Images

Product Details Angiopoietin 2 ELISA Kit Target details Application Details Handling References for Angiopoietin 2 Kit (ABIN624940) back to top
Supplier Images
ELISA image for Angiopoietin 2 ELISA Kit (ANGPT2) (ABIN624940) Angiopoietin 2 (ANGPT2) ELISA Kit