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Human Angiopoietin 2 ELISA Kit

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Antigen
Angiopoietin 2 (ANGPT2) ELISA Kits
  • AGPT2
  • Agpt2
  • ANG-2
  • Ang-2
  • ANG2
  • ang2
  • Ang2
  • ANGIOPOIETIN-2
  • ANGPT2
  • angpt2
Epitope
AA 19-496
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
24
15
10
10
8
5
4
3
3
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
156-10000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1672763
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN414354 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see 3
0.32693866 ABIN924873 Sandwich ELISA Cell Lysate, Serum, Plasma 94-6000 pg/mL 94 pg/mL Log in to see
0.32693866 ABIN1113387 Sandwich ELISA Serum, Plasma, Biological Fluids 46.875-3000 pg/mL 46.88 pg/mL Log in to see
0.32693866 ABIN454257 Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.32693866 ABIN1888483 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine 10-3000 pg/mL 10 pg/mL Log in to see
0.32693866 ABIN2708072 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 156-10000 pg/mL 156 pg/mL Log in to see
0.0012117105 ABIN365001 Sandwich ELISA Serum, Plasma, Tissue Homogenate 1.25-80 ng/mL 1.25 ng/mL Log in to see
0.0012117105 ABIN3032889 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4.1-3000 pg/mL 4.1 pg/mL Log in to see
0.0012117105 ABIN624940 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 7
0.0012117105 ABIN3198394 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012117105 ABIN1568361 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.781-50 ng/mL 0.781 ng/mL Log in to see
0.0012117105 ABIN1112556 Sandwich ELISA Serum, Plasma, Urine, Tissue Samples, Cell Culture Supernatant 8-150 pg/mL 8 pg/mL Log in to see
0.0012117105 ABIN2065294 Cell Lysate, Serum, Plasma 94-6000 pg/mL 94 pg/mL Log in to see
0.0012117105 ABIN2042346 Sandwich ELISA 6-10000 pg/mL 6 pg/mL Log in to see
0.0012117105 ABIN2683443 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Milk, Saliva, Serum 156-10000 pg/mL 156 pg/mL Log in to see
0.0012117105 ABIN2683444 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Samples, Urine 8-150 pg/mL 8 pg/mL Log in to see
0.0012117105 ABIN2115876 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.0012117105 ABIN2683445 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine Log in to see
0.0012117105 ABIN2541289 Serum, Plasma, Saliva, Biological Fluids 3-150 ng/L 3 ng/L Log in to see
0.0012117105 ABIN2541293 Sandwich ELISA Serum, Plasma, Saliva, Biological Fluids 12.5-800 pg/mL 12.5 pg/mL Log in to see

Top referenced Angiopoietin 2 ELISA Kit for ELISA

Similar Angiopoietin 2 ELISA Kits

Application / Reactivity Human
ELISA 23 ELISA Kits

General

Antigen Angiopoietin 2 (ANGPT2) ELISA Kits
Epitope AA 19-496
Reactivity Human
Kits with alternative reactivity to:
(24), (15), (10), (10), (8), (5), (4), (3), (3), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 156-10000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 3 references available
Supplier Log in to see

Product Details Angiopoietin 2 ELISA Kit

Target details Application Details Handling References for Angiopoietin 2 Kit (ABIN1672763) Images
Purpose For quantitative detection of human Angiopoietin-2 in cell culture supernates, serum, plasma(heparin, EDTA), saliva and human milk.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Milk
Detection Method Colorimetric
Specificity Natural and recombinant human Angiopoietin-2
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human Angiopoietin-2 antibod - 1
  • Lyophilized recombinant human Angiopoietin-2 standar - 10ng/tubex2
  • Biotinylated anti- human Angiopoietin-2 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: Y19-F496

Target details

Product Details Angiopoietin 2 ELISA Kit Application Details Handling References for Angiopoietin 2 Kit (ABIN1672763) Images back to top
Antigen
Alternative Name ANGPT2 (ANGPT2 ELISA Kit Abstract)
Background Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60 % homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
Gene ID 285
UniProt O15123
Pathways RTK Signaling

Application Details

Product Details Angiopoietin 2 ELISA Kit Target details Handling References for Angiopoietin 2 Kit (ABIN1672763) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human Angiopoietin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Angiopoietin-2 has been precoated onto 96-well plates. Standards(NSO, Y19-F496) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Angiopoietin-2 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Angiopoietin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Angiopoietin-2 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 1.08, Standard deviation: 0.049, CV(%): 4.5
  • Sample 2: n=16, Mean(ng/ml): 3.49, Standard deviation: 0.181, CV(%): 5.2
  • Sample 3: n=16, Mean(ng/ml): 7.01, Standard deviation: 0.407, CV(%): 5.8,
  • Sample 1: n=24, Mean(ng/ml): 1.05, Standard deviation: 0.071, CV(%): 6.8
  • Sample 2: n=24, Mean(ng/ml): 3.32, Standard deviation: 0.236, CV(%): 7.1
  • Sample 3: n=24, Mean(ng/ml): 6.67, Standard deviation: 0.5, CV(%): 7.5
Restrictions For Research Use only

Handling

Product Details Angiopoietin 2 ELISA Kit Target details Application Details References for Angiopoietin 2 Kit (ABIN1672763) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Angiopoietin 2 Kit (ABIN1672763)

Product Details Angiopoietin 2 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Ward, Grosios, Markham, Jones: "Genomic structures of the human angiopoietins show polymorphism in angiopoietin-2." in: Cytogenetics and cell genetics, Vol. 94, Issue 3-4, pp. 147-54, 2002

Kim, Kim, Ryu, Jung, Nah, Koh: "Characterization and expression of a novel alternatively spliced human angiopoietin-2." in: The Journal of biological chemistry, Vol. 275, Issue 24, pp. 18550-6, 2000

Maisonpierre, Suri, Jones, Bartunkova, Wiegand, Radziejewski, Compton, McClain, Aldrich, Papadopoulos, Daly, Davis, Sato, Yancopoulos: "Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis." in: Science (New York, N.Y.), Vol. 277, Issue 5322, pp. 55-60, 1997

Images

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Supplier Images
ELISA image for Angiopoietin 2 ELISA Kit (ANGPT2) (ABIN1672763) Human Angiopoietin-2 PicoKine ELISA Kit standard curve