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|Antigen||Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ARPC2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-ARPC2 AntibodyTarget Details ARPC2 Application Details Handling Images
|Specificity||This antibody detects ARPC2 / ARC34 at C-term.|
|Cross-Reactivity (Details)||Species reactivity (expected):Mouse, Rat, Canine (Dog) and CowSpecies reactivity (tested):Human|
|Purification||Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide|
|Immunogen||Peptide from C Terminus of the protein sequence according to NP_005722.1, NP_690601.1. Genename: ARPC2|
Target Details ARPC2Product Details anti-ARPC2 Antibody Application Details Handling Images back to top
|Alternative Name||ARPC2 / ARC34 (ARPC2 Antibody Abstract)|
|Background||ARPC2 is one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of ARPC2, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.Synonyms: Actin-related protein 2/3 complex subunit 2, Arp2/3 complex 34 kDa subunit, PRO2446, p34-ARC|
|Pathways||RTK Signaling, Regulation of Actin Filament Polymerization|
Application DetailsProduct Details anti-ARPC2 Antibody Target Details ARPC2 Handling Images back to top
Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.1-0.5 μg/mL. Approx 33-34 kDa band observed in Hela and Human SkeletalMuscle lysates (predicted MW of 34 kDa according to NP_005722). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Spleen shows cytoplasmstaining in some splenocytes in and around a T-cell area.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ARPC2 Antibody Target Details ARPC2 Application Details Images back to top
|Buffer||Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|