anti-Human BDNF antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended BDNF Antibody (supplied by: Log in to see )

Antigen
Brain-Derived Neurotrophic Factor (BDNF) Antibodies
  • bdnf-A
  • BDNF
  • Bdnf
  • bdnf
  • ANON2
  • BULN2
  • brain-derived neurotrophic factor
  • brain derived neurotrophic factor
  • brain-derived neurotrophic factor-like
  • bdnf
  • bdnf-A
  • BDNF
  • Bdnf
  • bdnf-b
  • LOC100356949
Reactivity
Human, Rat (Rattus)
320
132
114
18
16
13
12
7
6
5
5
4
3
3
2
2
Host
Mouse
225
116
11
11
10
5
Clonality
Monoclonal
Conjugate
This BDNF antibody is un-conjugated
31
20
13
7
7
6
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
288
150
110
69
66
54
54
23
13
11
9
4
4
4
2
2
1
1
1
1
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Catalog No. ABIN4283827
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.671394 ABIN1387788 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
13.671394 ABIN4283816 IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN392515 FACS IF IHC (p) WB Rabbit Ig AA 206-236, C-Term Log in to see Polyclonal 1
1 ABIN462499 IHC (p) Goat AA 183-194 Log in to see Polyclonal
1 ABIN4283815 IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN1732272 IHC (p) Goat Internal Region Log in to see Polyclonal
1 ABIN2617539 IHC (p) WB Rabbit IgG AA 129-247 Log in to see Polyclonal
1 ABIN1415121 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN4283826 FACS ICC IF IHC IHC (p) WB Mouse IgG2a kappa Log in to see 14C8-1D9
1 ABIN1397244 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN3030195 IHC (p) WB Rabbit IgG AA 129-247 Log in to see Polyclonal
1 ABIN4283844 FACS ICC IF IHC IHC (p) WB Alexa Fluor 700 Mouse IgG2a kappa Log in to see 14C8-1D9
1 ABIN4283837 FACS ICC IF IHC IHC (p) WB DyLight 755 Mouse IgG2a kappa Log in to see 14C8-1D9
1 ABIN4283830 FACS ICC IF IHC IHC (p) WB DyLight 350 Mouse IgG2a kappa Log in to see 14C8-1D9
1 ABIN4283831 FACS ICC IF IHC IHC (p) WB DyLight 405 Mouse IgG2a kappa Log in to see 14C8-1D9
1 ABIN4283833 FACS ICC IF IHC IHC (p) WB DyLight 488 Mouse IgG2a kappa Log in to see 14C8-1D9
1 ABIN4283834 FACS ICC IF IHC IHC (p) WB DyLight 550 Mouse IgG2a kappa Log in to see 14C8-1D9
1 ABIN4283836 FACS ICC IF IHC IHC (p) WB DyLight 680 Mouse IgG2a kappa Log in to see 14C8-1D9
1 ABIN4283842 FACS ICC IF IHC IHC (p) WB Alexa Fluor 647 Mouse IgG2a kappa Log in to see 14C8-1D9
1 ABIN4283841 FACS ICC IF IHC IHC (p) WB Alexa Fluor 488 Mouse IgG2a kappa Log in to see 14C8-1D9

General

Antigen Brain-Derived Neurotrophic Factor (BDNF) Antibodies
Reactivity Human, Rat (Rattus)
(320), (132), (114), (18), (16), (13), (12), (7), (6), (5), (5), (4), (3), (3), (2), (2)
Host Mouse
(225), (116), (11), (11), (10), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BDNF antibody is un-conjugated
(31), (20), (13), (7), (7), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(288), (150), (110), (69), (66), (54), (54), (23), (13), (11), (9), (4), (4), (4), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BDNF Antibody

Target Details BDNF Application Details Handling References for anti-BDNF antibody (ABIN4283827) Images
Purification Protein G purified
Immunogen Partial recombinant human BDNF protein (between amino acids 100-247) [UniProt P23560]
Clone 14C8-1D9
Isotype IgG2a kappa

Target Details BDNF

Product Details anti-BDNF Antibody Application Details Handling References for anti-BDNF antibody (ABIN4283827) Images back to top
Antigen
Alternative Name BDNF (BDNF Antibody Abstract)
Background Gene Symbol: BDNF
Gene ID 627
UniProt P23560
Research Area Cardiovascular, Alzheimer's Disease, Atherosclerosis, Hormones
Pathways RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-BDNF Antibody Target Details BDNF Handling References for anti-BDNF antibody (ABIN4283827) Images back to top
Application Notes Western Blot 2 μg/mL, Flow Cytometry 50 μg/mL, Immunohistochemistry 10-15 μg/mL, Immunocytochemistry/Immunofluorescence 20 μg/mL, Immunohistochemistry-Paraffin 10-15 μg/mLBDNF is a 247 amino acids long protein with predicted molecular weight of 27.8 kDa, however, it undergoes molecular processing/ cleavage as well as glycosylation and disulphide bond formation. Cleaved BDNF is ~14 kDa while the glycosylated form as well as the dimers may run at relatively higher molecular weight position in WB assay.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-BDNF Antibody Target Details BDNF Application Details References for anti-BDNF antibody (ABIN4283827) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-BDNF antibody (ABIN4283827)

Product Details anti-BDNF Antibody Target Details BDNF Application Details Handling Images back to top
Product cited in:

Lambert, Carlson, Formichella, Sappington, Ahlem, Calkins: "Oral Delivery of a Synthetic Sterol Reduces Axonopathy and Inflammation in a Rodent Model of Glaucoma." in: Frontiers in neuroscience, Vol. 11, pp. 45, 2017 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Rat (Rattus)).

Images

Product Details anti-BDNF Antibody Target Details BDNF Application Details Handling References for anti-BDNF antibody (ABIN4283827) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Brain-Derived Neurotrophic Factor (BDNF) antibody (ABIN4283827) Immunohistochemistry-Paraffin: BDNF Antibody (14C8.1D9) [NBP2-42215] - IHC analysis o...
Immunofluorescence (IF) image for anti-Brain-Derived Neurotrophic Factor (BDNF) antibody (ABIN4283827) Immunocytochemistry/Immunofluorescence: BDNF Antibody (14C8.1D9) [NBP2-42215] - BDNF ...
Western Blotting (WB) image for anti-Brain-Derived Neurotrophic Factor (BDNF) antibody (ABIN4283827) Western Blot: BDNF Antibody (14C8.1D9) [NBP2-42215] - WB analysis of a partial recomb...
 image for anti-Brain-Derived Neurotrophic Factor (BDNF) antibody (ABIN4283827) Flow (Intracellular): BDNF Antibody (14C8.1D9) [NBP2-42215] - U87MG cells were staine...