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anti-Human BDNF antibody for Neutralization

Recommended BDNF Antibody (supplied by: Log in to see )

Antigen
Brain-Derived Neurotrophic Factor (BDNF) Antibodies
  • bdnf-A
  • BDNF
  • Bdnf
  • bdnf
  • ANON2
  • BULN2
  • brain-derived neurotrophic factor
  • brain derived neurotrophic factor
  • brain-derived neurotrophic factor-like
  • bdnf
  • bdnf-A
  • BDNF
  • Bdnf
  • bdnf-b
  • LOC100356949
Reactivity
Human, Mouse (Murine), Rat (Rattus)
290
131
108
16
15
10
9
7
6
5
5
5
3
2
2
1
Host
Rabbit
232
84
10
10
9
5
Clonality
Polyclonal
Conjugate
This BDNF antibody is un-conjugated
32
21
14
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Neutralization (Neut), ELISA, Western Blotting (WB)
282
145
109
57
53
52
35
24
13
11
9
4
3
2
2
1
1
1
1
Supplier
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Available images

Catalog No. ABIN465693
$ 493.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN465696 ELISA IHC Neut WB Biotin Rabbit Log in to see Polyclonal
1 ABIN224400 Neut ELISA WB Mouse IgG1 Log in to see
1 ABIN2211862 Neut ELISA WB Mouse IgG1 Log in to see 7C20

Similar anti-BDNF Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 35 Antibodies 21 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 1 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Functional Studies (Func) 2 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 22 Antibodies 25 Antibodies
Immunofluorescence (IF) 22 Antibodies 24 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 55 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Western Blotting (WB) 100 Antibodies

General

Antigen Brain-Derived Neurotrophic Factor (BDNF) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(290), (131), (108), (16), (15), (10), (9), (7), (6), (5), (5), (5), (3), (2), (2), (1)
Host Rabbit
(232), (84), (10), (10), (9), (5)
Clonality
Polyclonal
Conjugate This BDNF antibody is un-conjugated
(32), (21), (14), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Neutralization (Neut), ELISA, Western Blotting (WB)
(282), (145), (109), (57), (53), (52), (35), (24), (13), (11), (9), (4), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-BDNF Antibody

Target Details BDNF Application Details Handling Images
Specificity Human BDNF
Purification Immunoaffinity purified
Immunogen E.coli derived recombinant Human BDNF.

Type of Immunogen: Cell extract

Target Details BDNF

Product Details anti-BDNF Antibody Application Details Handling Images back to top
Antigen
Alternative Name BDNF (BDNF Antibody Abstract)
Background Name/Gene ID: BDNF
Family: NGF Beta

Synonyms: BDNF, ANON2, Abrineurin, BULN2, Neurotrophin
Gene ID 627
UniProt P23560
Research Area Cardiovascular, Atherosclerosis, Alzheimer's Disease, Hormones
Pathways RTK Signaling

Application Details

Product Details anti-BDNF Antibody Target Details BDNF Handling Images back to top
Application Notes Approved: ELISA (0.5 - 2 μg/mL), IHC, Neut (0.54 - 0.81 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained U-2 OS and U-251 MG cells. The primary antibody was incubated at 2 μg/mL overnight at 4 °C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hBDNF (2 μg/mL), a concentration of 0.54-0.81 μg/mL of this antibody is required. ELISA: To detect hBDNF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BDNF (LS-C104729) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBDNF. Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-BDNF Antibody Target Details BDNF Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitute with sterile water.
Concentration Lot specific
Buffer Lyophilized, PBS, pH 7.2. Reconstitute with sterile water.
Handling Advice avoid freeze thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Images

Product Details anti-BDNF Antibody Target Details BDNF Application Details Handling back to top
Supplier Images
 image for anti-BDNF antibody (Brain-Derived Neurotrophic Factor) (ABIN465693) anti-Brain-Derived Neurotrophic Factor (BDNF) antibody