anti-Human BDNF antibody for Neutralization

Recommended BDNF Antibody (supplied by: Log in to see )

Antigen
Brain-Derived Neurotrophic Factor (BDNF) Antibodies
  • bdnf-A
  • BDNF
  • Bdnf
  • bdnf
  • ANON2
  • BULN2
  • brain-derived neurotrophic factor
  • brain derived neurotrophic factor
  • brain-derived neurotrophic factor-like
  • bdnf
  • bdnf-A
  • BDNF
  • Bdnf
  • bdnf-b
  • LOC100356949
Reactivity
Human, Mouse (Murine), Rat (Rattus)
320
132
113
18
16
13
12
7
6
5
5
4
3
3
2
2
Host
Rabbit
224
117
11
11
10
5
Clonality
Polyclonal
Conjugate
This BDNF antibody is un-conjugated
31
20
13
7
7
6
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Neutralization (Neut), ELISA, Western Blotting (WB)
288
149
110
70
67
55
55
23
13
11
9
4
4
3
2
2
1
1
1
1
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Catalog No. ABIN465693
$ 493.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN465696 ELISA IHC Neut WB Biotin Rabbit Log in to see Polyclonal
1 ABIN224400 Neut ELISA WB Mouse IgG1 Log in to see
1 ABIN2211862 Neut ELISA WB Mouse IgG1 Log in to see 7C20

General

Antigen Brain-Derived Neurotrophic Factor (BDNF) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(320), (132), (113), (18), (16), (13), (12), (7), (6), (5), (5), (4), (3), (3), (2), (2)
Host Rabbit
(224), (117), (11), (11), (10), (5)
Clonality
Polyclonal
Conjugate This BDNF antibody is un-conjugated
(31), (20), (13), (7), (7), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Neutralization (Neut), ELISA, Western Blotting (WB)
(288), (149), (110), (70), (67), (55), (55), (23), (13), (11), (9), (4), (4), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-BDNF Antibody

Target Details BDNF Application Details Handling Images
Specificity Human BDNF
Purification Immunoaffinity purified
Immunogen E.coli derived recombinant Human BDNF.

Type of Immunogen: Cell extract

Target Details BDNF

Product Details anti-BDNF Antibody Application Details Handling Images back to top
Antigen
Alternative Name BDNF (BDNF Antibody Abstract)
Background Name/Gene ID: BDNF
Family: NGF Beta

Synonyms: BDNF, ANON2, Abrineurin, BULN2, Neurotrophin
Gene ID 627
UniProt P23560
Research Area Cardiovascular, Atherosclerosis, Alzheimer's Disease, Hormones
Pathways RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-BDNF Antibody Target Details BDNF Handling Images back to top
Application Notes Approved: ELISA (0.5 - 2 μg/mL), IHC, Neut (0.54 - 0.81 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained U-2 OS and U-251 MG cells. The primary antibody was incubated at 2 μg/mL overnight at 4 °C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hBDNF (2 μg/mL), a concentration of 0.54-0.81 μg/mL of this antibody is required. ELISA: To detect hBDNF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BDNF (LS-C104729) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBDNF. Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-BDNF Antibody Target Details BDNF Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitute with sterile water.
Concentration Lot specific
Buffer Lyophilized, PBS, pH 7.2. Reconstitute with sterile water.
Handling Advice avoid freeze thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Images

Product Details anti-BDNF Antibody Target Details BDNF Application Details Handling back to top
Supplier Images
 image for anti-Brain-Derived Neurotrophic Factor (BDNF) antibody (ABIN465693) anti-Brain-Derived Neurotrophic Factor (BDNF) antibody