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anti-Human BDNF antibody for Western Blotting

Recommended BDNF Antibody (supplied by: Log in to see )

Antigen
Brain-Derived Neurotrophic Factor (BDNF) Antibodies
  • ANON2
  • bdnf
  • Bdnf
  • BDNF
  • bdnf-A
  • BULN2
Epitope
Middle Region
30
9
8
6
5
5
5
5
5
5
4
4
4
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
213
75
73
8
7
6
6
5
2
1
1
1
Host
Rabbit
214
32
8
8
8
5
Clonality
Polyclonal
Conjugate
This BDNF antibody is un-conjugated
29
20
12
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
206
133
64
23
14
13
12
11
9
6
4
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN2777093
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.117733724 ABIN392515 FACS IF IHC (p) WB Rabbit Ig AA 206-236, C-Term Log in to see Polyclonal 1
0.117733724 ABIN1724944 ICC ELISA WB Mouse IgG1 AA 19-248 Log in to see 3D9C5 1
0.117733724 ABIN1387788 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
0.117733724 ABIN1981374 IHC WB Rabbit IgG AA 206-236, C-Term Log in to see Polyclonal
0.117733724 ABIN1105511 WB Rabbit IgG Middle Region Log in to see Polyclonal
0.117733724 ABIN964574 ELISA WB Rabbit IgG Log in to see Polyclonal
0.117733724 ABIN3043096 WB Rabbit IgG AA 129-148, N-Term Log in to see Polyclonal
0.117733724 ABIN1871274 IHC WB Rabbit IgG Log in to see Polyclonal
0.117733724 ABIN393242 WB Mouse IgG2a kappa AA 1-248 Log in to see 1B10
0.117733724 ABIN1171949 IHC ELISA WB Biotin Rabbit IgG AA 129-247 Log in to see Polyclonal
0.117733724 ABIN2777092 WB Rabbit N-Term Log in to see Polyclonal
0.117733724 ABIN3021893 IHC WB Rabbit IgG Log in to see Polyclonal
0.117733724 ABIN1077869 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 129-247 Log in to see Polyclonal
0.117733724 ABIN634782 WB Rabbit Middle Region Log in to see Polyclonal
0.117733724 ABIN513711 IF IP ELISA WB Mouse IgG2a kappa AA 1-247, full length Log in to see 1B10
0.117733724 ABIN2157889 FACS IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.117733724 ABIN2421939 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.117733724 ABIN2404176 IHC WB Rabbit IgG Log in to see Polyclonal
0.117733724 ABIN448440 WB Rabbit IgG Middle Region Log in to see Polyclonal
0.117733724 ABIN513709 IF WB Mouse AA 1-255, full length Log in to see Polyclonal

Top referenced anti-BDNF antibody for Western Blotting

Similar anti-BDNF Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine)
Western Blotting (WB) 170 Antibodies 60 Antibodies 56 Antibodies 6 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Neutralization (Neut) 3 Antibodies
Immunoprecipitation (IP) 14 Antibodies 2 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies 12 Antibodies 11 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 62 Antibodies 22 Antibodies 24 Antibodies 1 Antibodies

General

Antigen Brain-Derived Neurotrophic Factor (BDNF) Antibodies
Epitope Middle Region
(30), (9), (8), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(213), (75), (73), (8), (7), (6), (6), (5), (2), (1), (1), (1)
Host Rabbit
(214), (32), (8), (8), (8), (5)
Clonality
Polyclonal
Conjugate This BDNF antibody is un-conjugated
(29), (20), (12), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(206), (133), (64), (23), (14), (13), (12), (11), (9), (6), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-BDNF Antibody

Target Details BDNF Application Details Handling References for anti-BDNF Antibody (ABIN2777093) Images
Blocking Peptide Blocking peptide for this product available: ABIN973798
Sequence EWVTAADKKT AVDMSGGTVT VLEKVPVSKG QLKQYFYETK CNPMGYTKEG
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %
Characteristics This is a rabbit polyclonal antibody against BDNF. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human BDNF

Target Details BDNF

Product Details anti-BDNF Antibody Application Details Handling References for anti-BDNF Antibody (ABIN2777093) Images back to top
Antigen
Alternative Name BDNF (BDNF Antibody Abstract)
Background BDNF is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression BDNF is reduced in both Alzheimer's and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders.The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.
Molecular Weight 27 kDa
Gene ID 627
NCBI Accession NP_001700, NM_001709
UniProt P23560
Research Area Cardiovascular, Atherosclerosis, Alzheimer's Disease, Hormones
Pathways RTK Signaling

Application Details

Product Details anti-BDNF Antibody Target Details BDNF Handling References for anti-BDNF Antibody (ABIN2777093) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 247 AA

Restrictions For Research Use only

Handling

Product Details anti-BDNF Antibody Target Details BDNF Application Details References for anti-BDNF Antibody (ABIN2777093) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-BDNF Antibody (ABIN2777093)

Product Details anti-BDNF Antibody Target Details BDNF Application Details Handling Images back to top
Product cited in:

Vassoler, White, Schmidt, Sadri-Vakili, Pierce: "Epigenetic inheritance of a cocaine-resistance phenotype." in: Nature neuroscience, Vol. 16, Issue 1, pp. 42-7, 2012

McCarthy, Zhang, Darnell, Sangrey, Yanagawa, Sadri-Vakili, Bhide: "Cocaine alters BDNF expression and neuronal migration in the embryonic mouse forebrain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 38, pp. 13400-11, 2011

Background publications

Hashimoto, Moriguchi, Yamashita, Mori, Nemoto, Okada, Hori, Noguchi, Kunugi, Ohnishi: "Dose-dependent effect of the Val66Met polymorphism of the brain-derived neurotrophic factor gene on memory-related hippocampal activity." in: Neuroscience research, Vol. 61, Issue 4, pp. 360-7, 2008

Images

Product Details anti-BDNF Antibody Target Details BDNF Application Details Handling References for anti-BDNF Antibody (ABIN2777093) back to top
Supplier Images
Western Blotting (WB) image for anti-BDNF antibody (Brain-Derived Neurotrophic Factor) (Middle Region) (ABIN2777093) anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody
Immunohistochemistry (IHC) image for anti-BDNF antibody (Brain-Derived Neurotrophic Factor) (Middle Region) (ABIN2777093) anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-BDNF antibody (Brain-Derived Neurotrophic Factor) (Middle Region) (ABIN2777093) anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody (Image 3)