anti-Human BDNF antibody for Western Blotting

Recommended BDNF Antibody (supplied by: Log in to see )

Antigen
Brain-Derived Neurotrophic Factor (BDNF) Antibodies
  • bdnf-A
  • BDNF
  • Bdnf
  • bdnf
  • ANON2
  • BULN2
  • brain-derived neurotrophic factor
  • brain derived neurotrophic factor
  • brain-derived neurotrophic factor-like
  • bdnf
  • bdnf-A
  • BDNF
  • Bdnf
  • bdnf-b
  • LOC100356949
Epitope
Middle Region
30
23
18
8
7
6
6
6
6
5
5
5
5
5
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
304
128
110
15
14
10
9
7
6
5
5
4
3
2
2
1
Host
Rabbit
222
104
11
10
9
5
Clonality
Polyclonal
Conjugate
This BDNF antibody is un-conjugated
30
20
13
6
6
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
282
150
108
70
67
55
42
23
13
11
9
4
4
2
2
1
1
1
1
Options
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Catalog No. ABIN2777093
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3442712 ABIN392515 FACS IF IHC (p) WB Rabbit Ig AA 206-236, C-Term Log in to see Polyclonal 1
3.3442712 ABIN1387788 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
3.3442712 ABIN1724944 ICC ELISA WB Mouse IgG1 AA 19-248 Log in to see 3D9C5 1
3.3442712 ABIN4283827 FACS ICC IF IHC IHC (p) WB Mouse IgG2a kappa Log in to see 14C8-1D9 1
3.3442712 ABIN3043096 WB Rabbit IgG AA 129-148, N-Term Log in to see Polyclonal
3.3442712 ABIN1981374 IHC WB Rabbit IgG AA 206-236, C-Term Log in to see Polyclonal
3.3442712 ABIN964574 ELISA WB Rabbit IgG Log in to see Polyclonal
3.3442712 ABIN1105511 WB Rabbit IgG Middle Region Log in to see Polyclonal
3.3442712 ABIN2777092 WB Rabbit N-Term Log in to see Polyclonal 1
3.3442712 ABIN1871274 IHC WB Rabbit IgG Log in to see Polyclonal
3.3442712 ABIN3197562 WB Rabbit Log in to see Polyclonal
3.3442712 ABIN393242 WB Mouse IgG2a kappa AA 1-248 Log in to see 1B10
3.3442712 ABIN4283817 ELISA ICC IF WB Mouse IgG1 Log in to see 3D9C5
3.3442712 ABIN1171949 IHC ELISA WB Biotin Rabbit IgG AA 129-247 Log in to see Polyclonal
3.3442712 ABIN1077869 ICC IHC IP WB Rabbit IgG AA 129-247 Log in to see Polyclonal
3.3442712 ABIN3021893 IHC WB Rabbit IgG Log in to see Polyclonal
3.3442712 ABIN448440 WB Rabbit Log in to see Polyclonal
3.3442712 ABIN3030196 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 206-236 Log in to see Polyclonal
3.3442712 ABIN5075282 ICC IF WB Rabbit IgG Log in to see Polyclonal
3.3442712 ABIN634782 WB Rabbit Middle Region Log in to see Polyclonal

Top referenced anti-BDNF antibody for Western Blotting

Similar anti-BDNF Antibodies

Application / Reactivity Human Rat (Rattus) Rabbit Dog (Canine) Pig (Porcine) Horse (Equine) Mouse (Murine)
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 231 Antibodies 86 Antibodies 96 Antibodies 13 Antibodies 14 Antibodies 10 Antibodies 10 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Neutralization (Neut) 4 Antibodies 2 Antibodies 2 Antibodies

General

Antigen Brain-Derived Neurotrophic Factor (BDNF) Antibodies
Epitope Middle Region
(30), (23), (18), (8), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(304), (128), (110), (15), (14), (10), (9), (7), (6), (5), (5), (4), (3), (2), (2), (1)
Host Rabbit
(222), (104), (11), (10), (9), (5)
Clonality
Polyclonal
Conjugate This BDNF antibody is un-conjugated
(30), (20), (13), (6), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(282), (150), (108), (70), (67), (55), (42), (23), (13), (11), (9), (4), (4), (2), (2), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-BDNF Antibody

Target Details BDNF Application Details Handling ProductDetails: References for anti-BDNF antibody (ABIN2777093) Images
Blocking Peptide Blocking peptide for this product available: ABIN973798
Sequence EWVTAADKKT AVDMSGGTVT VLEKVPVSKG QLKQYFYETK CNPMGYTKEG
Predicted Reactivity Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against BDNF. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human BDNF

Target Details BDNF

Product Details anti-BDNF Antibody Application Details Handling ProductDetails: References for anti-BDNF antibody (ABIN2777093) Images back to top
Antigen
Alternative Name BDNF (BDNF Antibody Abstract)
Background BDNF is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression BDNF is reduced in both Alzheimer's and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders.The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.
Alias Symbols: MGC34632
Protein Interaction Partner: UBC, AGO3, INPP5K, F11R, JUNB, APP, ELAVL1, Ntrk2, CADPS2, MBTPS1, SORT1, NOS3, NTF4, NTF3, ESR1, NCAM1, CPE, BDNF,
Protein Size: 247
Molecular Weight 27 kDa
Gene ID 627
NCBI Accession NM_001709, NP_001700
UniProt P23560
Research Area Cardiovascular, Atherosclerosis, Alzheimer's Disease, Hormones
Pathways RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-BDNF Antibody Target Details BDNF Handling ProductDetails: References for anti-BDNF antibody (ABIN2777093) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 247 AA

Restrictions For Research Use only

Handling

Product Details anti-BDNF Antibody Target Details BDNF Application Details ProductDetails: References for anti-BDNF antibody (ABIN2777093) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-BDNF antibody (ABIN2777093)

Product Details anti-BDNF Antibody Target Details BDNF Application Details Handling Images back to top
Product cited in:

McCarthy, Mueller, Cannon, Huizenga, Darnell, Bhide, Sadri-Vakili: "Prenatal Cocaine Exposure Alters BDNF-TrkB Signaling in the Embryonic and Adult Brain." in: Developmental neuroscience, Vol. 38, Issue 5, pp. 365-374, 2017

McCarthy, Bell, Cannon, Mueller, Huizenga, Sadri-Vakili, Fadool, Bhide: "Reversal Learning Deficits Associated with Increased Frontal Cortical Brain-Derived Neurotrophic Factor Tyrosine Kinase B Signaling in a Prenatal Cocaine Exposure Mouse Model." in: Developmental neuroscience, Vol. 38, Issue 5, pp. 354-364, 2016

Kight, McCarthy: "Sex differences and estrogen regulation of BDNF gene expression, but not propeptide content, in the developing hippocampus." in: Journal of neuroscience research, Vol. 95, Issue 1-2, pp. 345-354, 2016

Kasemeier-Kulesa, Morrison, Lefcort, Kulesa: "TrkB/BDNF signalling patterns the sympathetic nervous system." in: Nature communications, Vol. 6, pp. 8281, 2015

Vassoler, White, Schmidt, Sadri-Vakili, Pierce: "Epigenetic inheritance of a cocaine-resistance phenotype." in: Nature neuroscience, Vol. 16, Issue 1, pp. 42-7, 2012

McCarthy, Zhang, Darnell, Sangrey, Yanagawa, Sadri-Vakili, Bhide: "Cocaine alters BDNF expression and neuronal migration in the embryonic mouse forebrain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 38, pp. 13400-11, 2011

Background publications

Hashimoto, Moriguchi, Yamashita, Mori, Nemoto, Okada, Hori, Noguchi, Kunugi, Ohnishi: "Dose-dependent effect of the Val66Met polymorphism of the brain-derived neurotrophic factor gene on memory-related hippocampal activity." in: Neuroscience research, Vol. 61, Issue 4, pp. 360-7, 2008

Images

Product Details anti-BDNF Antibody Target Details BDNF Application Details Handling ProductDetails: References for anti-BDNF antibody (ABIN2777093) back to top
Supplier Images
Western Blotting (WB) image for anti-BDNF antibody (Brain-Derived Neurotrophic Factor) (Middle Region) (ABIN2777093) anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody
Immunohistochemistry (IHC) image for anti-BDNF antibody (Brain-Derived Neurotrophic Factor) (Middle Region) (ABIN2777093) anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-BDNF antibody (Brain-Derived Neurotrophic Factor) (Middle Region) (ABIN2777093) anti-Brain-Derived Neurotrophic Factor (BDNF) (Middle Region) antibody (Image 3)