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anti-Rabbit BDNF antibody for Immunohistochemistry (Frozen Sections)

Recommended BDNF Antibody (supplied by: Log in to see )

Antigen
Brain-Derived Neurotrophic Factor (BDNF) Antibodies
  • ANON2
  • bdnf
  • Bdnf
  • BDNF
  • bdnf-A
  • BULN2
Epitope
AA 16-183
30
9
8
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Rabbit
214
76
74
9
7
6
5
5
2
1
1
1
Host
Guinea Pig
215
32
8
8
8
4
Clonality
Polyclonal
Conjugate
This BDNF antibody is un-conjugated
29
20
12
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
206
132
65
22
14
12
11
11
9
6
4
3
2
2
1
1
1
1
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Catalog No. ABIN1858131
$ 376.20
Plus shipping costs $45.00

Similar anti-BDNF Antibodies

Application / Reactivity Rabbit
ELISA 3 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Western Blotting (WB) 4 Antibodies

General

Antigen Brain-Derived Neurotrophic Factor (BDNF) Antibodies
Epitope AA 16-183
(30), (9), (8), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rabbit
(214), (76), (74), (9), (7), (6), (5), (5), (2), (1), (1), (1)
Host Guinea Pig
(215), (32), (8), (8), (8), (4)
Clonality
Polyclonal
Conjugate This BDNF antibody is un-conjugated
(29), (20), (12), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(206), (132), (65), (22), (14), (12), (11), (11), (9), (6), (4), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-BDNF Antibody

Target Details BDNF Application Details Handling Images
Sequence MGHHHHHHSG SEF-MVISY FGCMKAAPMK EANVRGQGSL AYPGMRTHGT LESVNGPKTG SRGLTSLADT FEHVIEELLD EDQKVRPNEE NNKDADLYTS RVMLSSQVPL EPPLLFLLEE YKNYLDAANM SMRVRRHSDP ARRGELSVCD SISEWVTAAD KKTAVDMSGG TVTVLEKVPV SKG
Specificity It has been selected for its ability to recognize BDNF in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen BDNF (AA 16-183)
Isotype IgG

Target Details BDNF

Product Details anti-BDNF Antibody Application Details Handling Images back to top
Antigen
Alternative Name Brain Derived Neurotrophic Factor (BDNF) (BDNF Antibody Abstract)
Research Area Hormones
Pathways RTK Signaling

Application Details

Product Details anti-BDNF Antibody Target Details BDNF Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant BDNF (Met16~Gly183) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-BDNF Antibody Target Details BDNF Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-BDNF Antibody Target Details BDNF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BDNF antibody (Brain-Derived Neurotrophic Factor) (AA 16-183) (ABIN1858131) anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 16-183) antibody