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anti-Rat (Rattus) BDNF antibody for Immunocytochemistry

Recommended BDNF Antibody (supplied by: Log in to see )

Antigen
Brain-Derived Neurotrophic Factor (BDNF) Antibodies
  • ANON2
  • bdnf
  • Bdnf
  • BDNF
  • bdnf-A
  • BULN2
Epitope
AA 117-248
30
16
8
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
264
97
77
9
7
6
6
5
2
1
1
1
1
Host
Rabbit
218
78
8
8
8
5
Clonality
Polyclonal
Conjugate
This BDNF antibody is un-conjugated
31
21
14
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
249
133
88
45
32
31
30
14
13
11
4
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN1858129
$ 334.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009965406 ABIN409162 ICC IHC (fro) IF IHC (p) IHC WB Rabbit Log in to see Polyclonal
0.0009965406 ABIN4283826 FACS ICC IF IHC IHC (p) WB Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283827 FACS ICC IF IHC IHC (p) WB Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283828 FACS ICC IF IHC WB Allophycocyanin Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283832 FACS ICC IF IHC WB DyLight 405LS Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283833 FACS ICC IF IHC WB DyLight 488 Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283834 FACS ICC IF IHC WB DyLight 550 Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283836 FACS ICC IF IHC WB DyLight 680 Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283837 FACS ICC IF IHC WB DyLight 755 Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283839 FACS ICC IF IHC WB PE Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283840 FACS ICC IF IHC WB PerCP Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283841 FACS ICC IF IHC WB Alexa Fluor 488 Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283844 FACS ICC IF IHC WB Alexa Fluor 700 Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283829 FACS ICC IF IHC WB Biotin Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283831 FACS ICC IF IHC WB DyLight 405 Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283835 FACS ICC IF IHC WB DyLight 650 Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283838 FACS ICC IF IHC WB HRP Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283842 FACS ICC IF IHC WB Alexa Fluor 647 Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN4283843 FACS ICC IF IHC WB Alexa Fluor 405 Mouse IgG2a kappa Log in to see 14C8-1D9
0.0009965406 ABIN409227 ICC IF IHC IHC (p) WB Sheep IgG Log in to see Polyclonal

Similar anti-BDNF Antibodies

Application / Reactivity Rat (Rattus)
ELISA 26 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Functional Studies (Func) 2 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 23 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 44 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 78 Antibodies

General

Antigen Brain-Derived Neurotrophic Factor (BDNF) Antibodies
Epitope AA 117-248
(30), (16), (8), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(264), (97), (77), (9), (7), (6), (6), (5), (2), (1), (1), (1), (1)
Host Rabbit
(218), (78), (8), (8), (8), (5)
Clonality
Polyclonal
Conjugate This BDNF antibody is un-conjugated
(31), (21), (14), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(249), (133), (88), (45), (32), (31), (30), (14), (13), (11), (4), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-BDNF Antibody

Target Details BDNF Application Details Handling Images
Sequence MGHHHHHHSG SEF- NYLD AANMSMRVRR HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCRG IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRIDTSCV CTLTIKRG
Specificity It has been selected for its ability to recognize BDNF in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen BDNF (AA 117-248)
Isotype IgG

Target Details BDNF

Product Details anti-BDNF Antibody Application Details Handling Images back to top
Antigen
Alternative Name Brain Derived Neurotrophic Factor (BDNF) (BDNF Antibody Abstract)
Research Area Hormones
Pathways RTK Signaling

Application Details

Product Details anti-BDNF Antibody Target Details BDNF Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant BDNF (Asn117~Gly248) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-BDNF Antibody Target Details BDNF Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-BDNF Antibody Target Details BDNF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BDNF antibody (Brain-Derived Neurotrophic Factor) (AA 117-248) (ABIN1858129) anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 117-248) antibody