You are viewing an incomplete version of our website. Please click to reload the website as full version.

Goat BDNF ELISA Kit

Recommended BDNF Product (supplied by: Log in to see )

Antigen
Brain-Derived Neurotrophic Factor (BDNF) ELISA Kits
  • bdnf-A
  • BDNF
  • Bdnf
  • bdnf
  • ANON2
  • BULN2
  • brain-derived neurotrophic factor
  • brain derived neurotrophic factor
  • brain-derived neurotrophic factor-like
  • bdnf
  • bdnf-A
  • BDNF
  • Bdnf
  • bdnf-b
  • LOC100356949
Reactivity
Goat, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
33
21
19
8
7
7
7
6
6
3
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN4947403
$ 655.79
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN989524 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see

Similar BDNF ELISA Kits

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Horse (Equine) Rabbit Goat
ELISA 31 ELISA Kits 20 ELISA Kits 22 ELISA Kits 2 ELISA Kits 7 ELISA Kits 2 ELISA Kits

General

Antigen Brain-Derived Neurotrophic Factor (BDNF) ELISA Kits
Reactivity Goat, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
Kits with alternative reactivity to:
(33), (21), (19), (8), (7), (7), (7), (6), (6), (3), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Supplier Log in to see

Product Details BDNF ELISA Kit

Target details Application Details Handling Images
Purpose ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)
Sample Type Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate, Biological Fluids
Analytical Method Quantitative
Specificity

This assay has high sensitivity and excellent specificity for detection of Brain Derived Neurotrophic Factor (BDNF).
No significant cross-reactivity or interference between Brain Derived Neurotrophic Factor (BDNF) and analogues was observed.

Sensitivity 12.6 pg/mL

Target details

Product Details BDNF ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Brain Derived Neurotrophic Factor (BDNF ELISA Kit Abstract)
Pathways RTK Signaling

Application Details

Product Details BDNF ELISA Kit Target details Handling Images back to top
Assay Time 3 h
Plate Pre-coated
Protocol The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Derived Neurotrophic Factor (BDNF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Derived Neurotrophic Factor (BDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Derived Neurotrophic Factor (BDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Brain Derived Neurotrophic Factor (BDNF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Procedure 1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.
Assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions For Research Use only

Handling

Product Details BDNF ELISA Kit Target details Application Details Images back to top
Handling Advice

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage 4 °C