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|Antigen||Brain-Derived Neurotrophic Factor (BDNF) ELISA Kits|
|Reactivity||Goat, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus) Alternatives|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||31.2-2000 pg/mL|
|Minimum Detection Limit||31.2 pg/mL|
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Product Details BDNF ELISA KitTarget details Application Details Handling Images
|Purpose||ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)|
|Sample Type||Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate, Biological Fluids|
This assay has high sensitivity and excellent specificity for detection of Brain Derived Neurotrophic Factor (BDNF).
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|Alternative Name||Brain Derived Neurotrophic Factor (BDNF ELISA Kit Abstract)|
Application DetailsProduct Details BDNF ELISA Kit Target details Handling Images back to top
|Assay Time||3 h|
|Protocol||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Derived Neurotrophic Factor (BDNF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Derived Neurotrophic Factor (BDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Derived Neurotrophic Factor (BDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Brain Derived Neurotrophic Factor (BDNF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.|
1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested 20 times on one plate, respectively.
|Restrictions||For Research Use only|
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.