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Rat (Rattus) Betacellulin ELISA Kit

Recommended Betacellulin Product (supplied by: Log in to see )

Antigen
Betacellulin (BTC) ELISA Kits
  • Bcn
  • BTC
  • LOC100228121
  • probetacellulin
Epitope
AA 32-111
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
18
13
8
4
3
3
3
3
2
2
2
1
Methode Type
Sandwich ELISA
Detection Range
7.8-500 pg/mL
Minimum Detection Limit
7.8 pg/mL
Application
ELISA
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Catalog No. ABIN1889437
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN1113702 Sandwich ELISA Serum, Plasma, Biological Fluids 9.38-600 pg/mL 9.38 pg/mL Log in to see
0.0012155735 ABIN771529 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 25-500 pg/mL 25 pg/mL Log in to see
0.0012155735 ABIN368146 Serum, Plasma, Tissue Homogenate 5000-78.13 pg/mL 5000 pg/mL Log in to see
0.0012155735 ABIN1568689 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 9.375-600 pg/mL 9.375 pg/mL Log in to see
0.0012155735 ABIN2748894 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.0012155735 ABIN2683572 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.0012155735 ABIN2067324 Sandwich ELISA Log in to see
0.0012155735 ABIN2945574 9.375-600 pg/mL 9.375 pg/mL Log in to see

Top referenced Betacellulin ELISA Kit for ELISA

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Application / Reactivity Rat (Rattus)
ELISA 9 ELISA Kits

General

Antigen Betacellulin (BTC) ELISA Kits
Epitope AA 32-111
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(18), (13), (8), (4), (3), (3), (3), (3), (2), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 7.8-500 pg/mL
Minimum Detection Limit 7.8 pg/mL
Application ELISA
Pubmed 3 references available
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Product Details Betacellulin ELISA Kit

Target details Application Details Handling References for Betacellulin Kit (ABIN1889437) Images
Purpose For quantitative detection of rat BTC in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant rat BTC
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- rat BTC antibod - 1
  • Lyophilized recombinant rat BTC standar - 10ng/tubex2
  • Biotinylated anti- rat BTC antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: D32-Y111

Target details

Product Details Betacellulin ELISA Kit Application Details Handling References for Betacellulin Kit (ABIN1889437) Images back to top
Antigen
Alternative Name BTC (BTC ELISA Kit Abstract)
Background Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
Gene ID 64022
UniProt Q9JJM4
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details Betacellulin ELISA Kit Target details Handling References for Betacellulin Kit (ABIN1889437) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol rat BTC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BTC has been precoated onto 96-well plates. Standards(E.coli, D32-Y111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BTC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat BTC amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL rat BTC standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat BTC standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 95, Standard deviation: 3.61, CV(%): 3.8
  • Sample 2: n=16, Mean(pg/ml): 178, Standard deviation: 7.3, CV(%): 4.1
  • Sample 3: n=16, Mean(pg/ml): 362, Standard deviation: 16.29, CV(%): 4.5,
  • Sample 1: n=24, Mean(pg/ml): 105, Standard deviation: 4.62, CV(%): 4.4
  • Sample 2: n=24, Mean(pg/ml): 241, Standard deviation: 12.53, CV(%): 5.2
  • Sample 3: n=24, Mean(pg/ml): 413, Standard deviation: 23.13, CV(%): 5.6
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Betacellulin Kit (ABIN1889437)

Product Details Betacellulin ELISA Kit Target details Application Details Handling Images back to top
Background publications

Park, Su, Ariga, Law, Jin, Conti: "EGF-like growth factors as mediators of LH action in the ovulatory follicle." in: Science (New York, N.Y.), Vol. 303, Issue 5658, pp. 682-4, 2004

Watanabe, Shintani, Nakata, Shing, Folkman, Igarashi, Sasada: "Recombinant human betacellulin. Molecular structure, biological activities, and receptor interaction." in: The Journal of biological chemistry, Vol. 269, Issue 13, pp. 9966-73, 1994

Sasada, Ono, Taniyama, Shing, Folkman, Igarashi: "Cloning and expression of cDNA encoding human betacellulin, a new member of the EGF family." in: Biochemical and biophysical research communications, Vol. 190, Issue 3, pp. 1173-9, 1993

Images

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Supplier Images
ELISA image for Betacellulin ELISA Kit (BTC) (ABIN1889437) Rat BTC PicoKine ELISA Kit standard curve